School of Environment and Life Sciences, University of Salford, Peel Building, The Crescent, Salford, Greater Manchester M5 4WT, UK.
Mol Ecol Resour. 2008 Nov;8(6):1326-8. doi: 10.1111/j.1755-0998.2008.02304.x. Epub 2008 Aug 29.
Within the anuran amphibians, dendrobatoids (poison-arrow frogs) are renowned for their parental care, but the lack of highly variable DNA markers so far precluded precise and comprehensive measurements of their genetic mating system. Here we present 10 polymorphic microsatellites for Allobates femoralis, a widespread dendrobatoid from the Amazon basin. In 24 field-collected individuals, we found between seven and 15 alleles per locus, without deviations from Hardy-Weinberg equilibrium or linkage disequilibrium. The loci will be employed to quantify reproductive success in view of male territoriality and female mate choice, as well as to determine the fine-scale genetic structure of local populations.
在有尾两栖类动物中，蟾蜍科（毒镖蛙）以其亲代照料而闻名，但由于缺乏高度可变的 DNA 标记，迄今为止，它们的遗传交配系统无法得到精确和全面的测量。在这里，我们为亚马逊流域广泛分布的蟾蜍科物种 Allobates femoralis 提供了 10 个多态性微卫星标记。在 24 个野外采集的个体中，我们在每个位点发现了 7 到 15 个等位基因，没有偏离哈迪-温伯格平衡或连锁不平衡。这些位点将被用于量化雄性领域性和雌性配偶选择下的繁殖成功，以及确定局部种群的精细遗传结构。
