Department of Ecology and Evolutionary Biology, Corson Hall, Cornell University, Ithaca, NY 14853, USA, Department of Biology, Duke University, Durham, NC 27708, USA.
Mol Ecol Resour. 2008 Mar;8(2):434-6. doi: 10.1111/j.1471-8286.2007.01983.x.
We developed nine polymorphic microsatellite loci for evening primrose (Oenothera biennis). These loci have two to 18 alleles per locus and observed heterozygosities ranging from 0 to 0.879 in a sample of 34 individuals. In a pattern consistent with the functionally asexual reproductive system of this species, 17/36 pairs of loci revealed significant linkage disequilibrium and three loci showed significant deviations from Hardy-Weinberg equilibrium. The loci will be informative in identifying genotypes in multigenerational field studies to assess changes in genotype frequencies.
我们为月见草（Oenothera biennis）开发了九个多态性微卫星位点。在 34 个个体的样本中，每个位点有 2 到 18 个等位基因，观察到的杂合度从 0 到 0.879。在与该物种功能上无性繁殖系统一致的模式下，36 对位点中的 17 对显示出显著的连锁不平衡，3 个位点显示出与哈迪-温伯格平衡的显著偏离。这些位点将在识别多代田间研究中的基因型方面提供信息，以评估基因型频率的变化。
