永久性遗传资源：普通月见草（Oenothera biennis）中多态性微卫星位点的分离与鉴定。
PERMANENT GENETIC RESOURCES: Isolation and characterization of polymorphic microsatellite loci in common evening primrose (Oenothera biennis).
机构信息
Department of Ecology and Evolutionary Biology, Corson Hall, Cornell University, Ithaca, NY 14853, USA, Department of Biology, Duke University, Durham, NC 27708, USA.
出版信息
Mol Ecol Resour. 2008 Mar;8(2):434-6. doi: 10.1111/j.1471-8286.2007.01983.x.
We developed nine polymorphic microsatellite loci for evening primrose (Oenothera biennis). These loci have two to 18 alleles per locus and observed heterozygosities ranging from 0 to 0.879 in a sample of 34 individuals. In a pattern consistent with the functionally asexual reproductive system of this species, 17/36 pairs of loci revealed significant linkage disequilibrium and three loci showed significant deviations from Hardy-Weinberg equilibrium. The loci will be informative in identifying genotypes in multigenerational field studies to assess changes in genotype frequencies.
我们为月见草（Oenothera biennis）开发了九个多态性微卫星位点。在 34 个个体的样本中，每个位点有 2 到 18 个等位基因，观察到的杂合度从 0 到 0.879。在与该物种功能上无性繁殖系统一致的模式下，36 对位点中的 17 对显示出显著的连锁不平衡，3 个位点显示出与哈迪-温伯格平衡的显著偏离。这些位点将在识别多代田间研究中的基因型方面提供信息，以评估基因型频率的变化。
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