Department of Animal Ecology, Ecology Building, Lund University, SE-22362 Lund, Sweden.
Mol Ecol Resour. 2008 Nov;8(6):1367-70. doi: 10.1111/j.1755-0998.2008.02299.x.
We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species.
我们测试了 27 个微卫星基因座在壁虎科的蜥蜴属和壁虎属中的跨物种扩增。我们分别在前者和后者中检测到 11 个和 15 个多态性基因座。在来自每个物种的单个种群的更大个体样本中，10 个多态性基因座中 P. gaigeae 的等位基因数从 5 到 23 不等，9 个多态性基因座中 P. hispanica 的等位基因数从 4 到 13 不等。两个基因座在 P. hispanica 中偏离哈迪-温伯格平衡。27 个基因座中的 11 到 16 个也在另外三个壁虎物种中成功扩增。
