用于观赏鱼盘丽鱼的微卫星标记的分离和特征分析，以及在其他英雄丽鱼属慈鲷物种中的跨物种扩增。
Isolation and characterization of microsatellite markers for the ornamental discus fish Symphysodon discus and cross-species amplification in other Heroini cichlid species.
机构信息
Departamento de Biologia, Laboratório de Evolução e Genética Animal, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jordão Ramos, 3000, 69077-000, Manaus, Amazonas, Brazil, Biology Department, University of Puerto Rico - Rio Piedras, San Juan, PR 00931, Puerto Rico, Centro de Apoio Muldisciplinar, Laboratório de Tecnologias de DNA, Universidade Federal do Amazonas, Av. Rodrigo Octávio Jordão Ramos, 3000, 69077-000, Manaus, Amazonas, Brazil.
出版信息
Mol Ecol Resour. 2008 Nov;8(6):1451-3. doi: 10.1111/j.1755-0998.2008.02200.x. Epub 2008 Sep 15.
The discus fishes (Symphysodon spp.) are economically important ornamental species. Thirteen microsatellite markers were developed from a CT(12) - and CA(12) -enriched whole genomic DNA library of Symphysodon discus. Allelic variability was tested on 44 individuals of two species (S. discus and S. aequifasciatus). Allelic richness ranged from two to 11 alleles per locus and observed heterozygosities from 0.083 to 0.998. All loci were at Hardy-Weinberg equilibrium, and no pair of loci showed linkage disequilibrium within a species. Cross-species amplification was also successfully performed in the Neotropical cichlids Uaru amphiacanthoides, Hoplarchus psittacus, Hypselecara coryphaenoides, Pterophyllum sp., Mesonauta sp. and Heros sp.
盘丽鱼（Symphysodon spp.）是具有经济价值的重要观赏鱼类。本研究从盘丽鱼基因组 DNA 文库中筛选到 13 对微卫星标记，并用这 13 对微卫星标记对盘丽鱼和阿莲卡蓝袖鱼两个物种的 44 个个体进行了遗传多样性分析。结果显示，每个位点的等位基因数为 2-11 个，观测杂合度为 0.083-0.998。所有位点均处于哈迪-温伯格平衡状态，且同一物种内的任意两个位点间均不存在连锁不平衡。这些微卫星标记还可成功地用于其他热带慈鲷科鱼类的遗传分析，如 Uaru amphiacanthoides、Hoplarchus psittacus、Hypselecara coryphaenoides、Pterophyllum sp.、Mesonauta sp. 和 Heros sp.
Zotero 插件