加拉诺氏长足蛛 12 个二核苷酸微卫星位点的分离与特征。

Isolation and characterization of 12 dinucletiotide microsatellite loci in Paratrechalea galianoae (Araneae, Trechaleidae), a nuptial gift-spider.

机构信息

Programa de Pós-Graduação em Genética e Biologia Molecular, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil Faculdade de Biociências, PUCRS. Av. Ipiranga 6681, 90619-900 Porto Alegre, RS, Brazil.

出版信息

Mol Ecol Resour. 2009 Mar;9(2):539-41. doi: 10.1111/j.1755-0998.2008.02338.x. Epub 2009 Jan 20.

DOI:10.1111/j.1755-0998.2008.02338.x

PMID:21564686

Abstract

Twelve microsatellite loci from the Neotropical spider Paratrechalea galianoae were isolated from a microsatellite-enriched genomic library, and were tested in a sample of 27 individuals from a population that occurs in Brazil. All loci were polymorphic and three to 14 (mean 7.58) alleles per locus were identified. The observed and expected heterozygosities per locus ranged from 0.417 to 0.885 and from 0.595 to 0.878, respectively. Two loci deviated significantly from Hardy-Weinberg equilibrium and one pair showed evidence of linkage disequilibrium. These loci are being used to study the population structure and genetic diversity in P. galianoae.

摘要

从分布在巴西的一个种群中，分离出了 27 个个体的微卫星富集基因组文库中的 12 个新热带蜘蛛 Paratrechalea galianoae 的微卫星位点。所有位点均为多态性，每个位点可检测到 3 到 14 个（平均 7.58 个）等位基因。每个位点的观测杂合度和期望杂合度范围分别为 0.417 到 0.885 和 0.595 到 0.878。有两个位点偏离哈迪-温伯格平衡显著，一对位点显示连锁不平衡的证据。这些位点正在用于研究 P. galianoae 的种群结构和遗传多样性。