在白腰缟鰕虎鱼（Pterogobius zonoleucus）中分离和特征分析 11 个多态性微卫星位点，并在蛇缟鰕虎鱼（P. elapoides）中进行跨物种扩增。

Isolation and characterization of 11 polymorphic microsatellite loci in the whitegirdled goby (Pterogobius zonoleucus) and cross-species amplification in the serpentine goby (P. elapoides).

机构信息

Department of Marine Bioscience, Faculty of Biotechnology, Fukui Prefectural University, Gakuen-cho, Obama 1-1, Fukui 917-0003, Japan.

出版信息

Mol Ecol Resour. 2009 Mar;9(2):610-2. doi: 10.1111/j.1471-8286.2009.00228_9_2.x. Epub 2008 Nov 12.

DOI:10.1111/j.1471-8286.2009.00228_9_2.x

PMID:21564706

Abstract

Eleven polymorphic microsatellite markers were developed from a (CA)(n) -enrichment library of the whitegirdled goby (Pterogobius zonoleucus). Polymorphism at these loci ranged from 2 to 12 alleles, and observed and expected heterozygosities from 0.05 to 0.90 and from 0.05 and 0.86, respectively. All loci conformed to Hardy-Weinberg equilibrium, with no significant linkage disequilibrium between all locus pairs. Cross-species amplification tests were successful in P. elapoides, and most loci were polymorphic. These microsatellite markers will be useful in further population genetic studies of both species.

摘要

从白腰带鰕虎鱼（Pterogobius zonoleucus）的（CA）（n）富集文库中开发了 11 个多态性微卫星标记。这些位点的多态性范围从 2 到 12 个等位基因，观察到的和预期的杂合度分别从 0.05 到 0.90 和从 0.05 到 0.86。所有位点均符合哈迪-温伯格平衡，所有位点对之间没有显著的连锁不平衡。在 P. elapoides 中成功进行了跨物种扩增测试，并且大多数位点都是多态的。这些微卫星标记将有助于进一步研究这两个物种的种群遗传。