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用于采采蝇 Glossina fuscipes fuscipes(双翅目:Glossinidae)的多态微卫星标记,一种人类非洲锥虫病的媒介。

Polymorphic microsatellite markers for the tsetse fly Glossina fuscipes fuscipes (Diptera: Glossinidae), a vector of human African trypanosomiasis.

机构信息

Department of Ecology and Evolutionary Biology, Yale University, 21 Sachem Street, New Haven, CT 06511, USA, National Livestock Resources Research Institute, PO Box 96, Tororo, Uganda, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, Insect Pest Control Sub-Programme, International Atomic Energy Agency, A-1400 Vienna, Austria, Vector Research Group, Liverpool School of Tropical Medicine, Liverpool L3 5QA, UK.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1506-8. doi: 10.1111/j.1755-0998.2008.02328.x.

DOI:10.1111/j.1755-0998.2008.02328.x
PMID:21586090
Abstract

Our understanding of Glossina fuscipes fuscipes, a major vector of sleeping sickness, has been severely constrained by a lack of genetic markers for mapping and population genetic studies. Here we present 10 newly developed microsatellite loci for this tsetse species. Heterozygosity levels in Moyo, an Ugandan population, averaged 0.57, with only two loci showing very low heterozygosity. Five loci carried more than six alleles. Together with five recently published microsatellite loci, this brings the number of available microsatellite loci for this species to 15. Their availability will greatly facilitate future studies on the genetics of this important human disease vector.

摘要

我们对采采蝇( Glossina fuscipes fuscipes )的了解受到严重限制,因为缺乏用于绘图和种群遗传研究的遗传标记。在这里,我们为这个采采蝇物种介绍了 10 个新开发的微卫星基因座。在乌干达的莫约地区,种群的平均杂合度为 0.57,只有两个位点显示出非常低的杂合度。有五个位点携带了超过六个等位基因。再加上最近公布的五个微卫星基因座,这使得该物种的可用微卫星基因座数量达到了 15 个。这些基因座的可用性将极大地促进对这种重要的人类疾病媒介的遗传学的未来研究。

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