Zhirnov O P
D.I. Ivanovsky Institute of Virology, Moscow, USSR.
Virology. 1990 May;176(1):274-9. doi: 10.1016/0042-6822(90)90253-n.
Enveloped viruses, of which the orthomyxo- and paramyxoviruses are members, are known to be uncoated by nonionic detergents in a salt concentration-dependent manner. In this study we have shown that detergent uncoating of myxoviruses depends not only on salt concentration but also on pH. Treatment of orthomyxoviruses with Nonidet-P40 or Triton N-101 at low salt concentrations results in solubilization of surface virion glycopolypeptides in alkaline and neutral pH (9.0-6.5), but in acidic pH (6.0-5.0) the viral matrix protein M1 is also removed, and the viral ribonucleoprotein complex is released. Conversely, the paramyxovirus matrix protein M is more completely solubilized in alkaline pH (pH 9.0) than in neutral and acidic pH 7.4-5.0. The described pH-dependent differences are discussed in terms of orthomyxo- and paramyxovirus uncoating in target cells.
被膜病毒，正黏液病毒和副黏液病毒都属于这类病毒，已知它们会以盐浓度依赖的方式被非离子去污剂脱去包膜。在本研究中，我们已经表明黏液病毒的去污剂脱壳不仅取决于盐浓度，还取决于pH值。在低盐浓度下用诺乃洗涤剂-P40或吐温N-101处理正黏液病毒，会导致表面病毒粒子糖多肽在碱性和中性pH值（9.0 - 6.5）下溶解，但在酸性pH值（6.0 - 5.0）下，病毒基质蛋白M1也会被去除，病毒核糖核蛋白复合体被释放。相反，副黏液病毒基质蛋白M在碱性pH值（pH 9.0）下比在中性和酸性pH值7.4 - 5.0下更完全地溶解。根据正黏液病毒和副黏液病毒在靶细胞中的脱壳情况对所描述的pH值依赖性差异进行了讨论。
