Kollenda M C, Vollmar A M, McEnroe G A, Gerbes A L
Department of Medicine II, Klinikum Grosshadern, Munich, Federal Republic of Germany.
Am J Physiol. 1990 Apr;258(4 Pt 2):R1084-8. doi: 10.1152/ajpregu.1990.258.4.R1084.
The present study determined the presence of two types of binding sites for atrial natriuretic factor (ANF), the B and C receptor, on rat glomerular membranes. The effect of short-term salt loading and dehydration on these two receptor populations was investigated consecutively. Salt-loaded rats did not show significant changes in plasma ANF concentrations or in the number of ANF binding sites. Water-deprived rats presented significantly lower plasma ANF concentrations (22.0 +/- 1.9 vs. 34.4 +/- 3.8 fmol/ml, P less than 0.01) and an increase in total receptor density (1,860 +/- 398 vs. 987 +/- 143 fmol/mg protein) as compared with the control group. Differentiation of both receptor populations showed that it was the C receptors that accounted for this increase (1,772 +/- 369 vs. 901 +/- 151 fmol/mg protein, P less than 0.05), whereas B-receptor density was unchanged (89 +/- 31 vs. 87 +/- 44 fmol/mg protein). These data suggest that C receptors for ANF are affected by changes of body fluid volume.
本研究测定了大鼠肾小球膜上心房利钠因子（ANF）的两种结合位点，即B受体和C受体的存在情况。连续研究了短期盐负荷和脱水对这两种受体群体的影响。盐负荷大鼠的血浆ANF浓度或ANF结合位点数量未显示出显著变化。与对照组相比，缺水大鼠的血浆ANF浓度显著降低（22.0±1.9对34.4±3.8 fmol/ml，P<0.01），总受体密度增加（1860±398对987±143 fmol/mg蛋白质）。对两种受体群体的区分表明，是C受体导致了这种增加（1772±369对901±151 fmol/mg蛋白质，P<0.05），而B受体密度未改变（89±31对87±44 fmol/mg蛋白质）。这些数据表明，ANF的C受体受体液量变化的影响。
