Gauquelin G, Thibault G, Cantin M, Schiffrin E L, Garcia R
Laboratory of Experimental Hypertension and Vasoactive Peptides, Clinical Research Institute of Montreal, Canada.
Am J Physiol. 1988 Oct;255(4 Pt 2):F621-5. doi: 10.1152/ajprenal.1988.255.4.F621.
Atrial natriuretic factor (ANF) concentration in atria and plasma was investigated in relation to the density and affinity of renal glomerular ANF receptors during water deprivation and rehydration in the rat. Immunoreactive (IR) ANF-(99-126) was lower during water deprivation (4.2 +/- 0.5 fmol/ml) than in normally hydrated animals (10 +/- 1.5 fmol/ml); NH2-terminal IR ANF-(1-98) was also lower in water-deprived rats (75 +/- 2.4 fmol/ml) than in the controls (708 +/- 105 fmol/ml). These decreased plasma COOH- and NH2-terminal ANF concentrations were accompanied by significantly higher hematocrit, serum osmolality, and Na+ values. The renal glomerular ANF receptor population was greater in water-deprived animals than a control animals. After rehydration, IR ANF was elevated within 1 h and reached control values after 6 h. Hematocrit, serum osmolality, and Na+ did not normalize until 48, 6, and 24 h, respectively. The renal glomerular ANF receptor population, which was markedly higher in water-deprived rats, gradually declined after rehydration, so that no difference was observed with the control group 24 h later.
在大鼠限水及复水过程中，研究了心房及血浆中心房利钠因子（ANF）的浓度与肾小球ANF受体密度和亲和力的关系。限水期间免疫反应性（IR）ANF-(99 - 126)低于正常水合动物（分别为4.2±0.5 fmol/ml和10±1.5 fmol/ml）；限水大鼠中NH2末端IR ANF-(1 - 98)也低于对照组（分别为75±2.4 fmol/ml和708±105 fmol/ml）。血浆COOH末端和NH2末端ANF浓度降低的同时，血细胞比容、血清渗透压和Na+值显著升高。限水动物的肾小球ANF受体数量多于对照动物。复水后，IR ANF在1小时内升高，6小时后达到对照值。血细胞比容、血清渗透压和Na+分别在48小时、6小时和24小时后恢复正常。限水大鼠中明显较高的肾小球ANF受体数量在复水后逐渐下降，24小时后与对照组无差异。
