利用表面增强拉曼光谱进行定量无标记 RNA 检测。
Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy.
机构信息
Université Bordeaux 1-CNRS, Chimie et Biologie des Membranes et des Nanoobjets, 2 rue Robert Escarpit, 33607 Pessac, France.
出版信息
Chem Commun (Camb). 2011 Jul 14;47(26):7425-7. doi: 10.1039/c1cc11925g. Epub 2011 May 17.
Surface-Enhanced Raman Spectroscopy (SERS) was performed to detect label-free RNA. We defined conditions which make it possible to probe the four bases of RNA, in single strands of polyadenosine (pA), polyuridine (pU), polycytosine (pC) and polyguanosine (pG). We therefore present below a quantitative analysis of mixtures of non-hybridized single strands, based on the deconvolution of the SERS mixture spectrum into the relative contributions of the SERS spectra of each constituent.
表面增强拉曼光谱（SERS）被用于检测无标记的 RNA。我们确定了条件，使其可以探测 RNA 的四种碱基，即聚腺苷酸（pA）、聚尿苷酸（pU）、聚胞苷酸（pC）和聚鸟苷酸（pG）的单链。因此，我们根据混合物的 SERS 光谱的解卷积为每个组成部分的 SERS 光谱的相对贡献，给出了非杂交单链混合物的定量分析。
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