School of Chemistry and Environment, Beijing University of Aeronautics and Astronautics, Beijing, 100191, China.
Biosens Bioelectron. 2011 Aug 15;26(12):4828-31. doi: 10.1016/j.bios.2011.05.042. Epub 2011 Jun 1.
A novel surface-enhanced Raman scattering (SERS) approach for accurate quantification of mononucleotides of deoxyribonucleic acid (DNA) is described. Reproducible SERS measurement was achieved by using isotopically labeled internal standard. By measuring the SERS spectra of mononucleotides and its isotope internal standard in combination with multivariate data analysis, the method was successfully applied to quantify mononucleotides. The independent validation of analyte concentrations gave a standard deviation of within 2%, which is comparable to HPLC result. Finally, a mixture of four mononucleotides of DNA was prepared to explore the possibility of quantifying the concentration of label-free, sequence-specific DNA strands by this approach. As compared to liquid chromatography/mass spectrometry (LC/MS), our method can be similarly precise but the SERS measurement is simple, rapid and potentially cheap.
本文描述了一种用于准确定量脱氧核糖核酸（DNA）单核苷酸的新型表面增强拉曼散射（SERS）方法。通过使用同位素标记的内标，实现了可重复的 SERS 测量。通过测量单核苷酸及其同位素内标物的 SERS 光谱，并结合多元数据分析，该方法成功地应用于定量单核苷酸。对分析物浓度的独立验证给出了 2%以内的标准偏差，这与 HPLC 结果相当。最后，制备了四种 DNA 单核苷酸的混合物，以探索通过这种方法定量无标记、序列特异性 DNA 链浓度的可能性。与液相色谱/质谱（LC/MS）相比，我们的方法可以同样精确，但 SERS 测量简单、快速且具有潜在的成本效益。
