Institute of Advanced Materials, Nanjing University of Posts and Telecommunications , Nanjing 210046, People's Republic of China.
Biomacromolecules. 2013 Jun 10;14(6):1727-31. doi: 10.1021/bm301221t. Epub 2013 May 3.
Electrically conducting polymers (CPs) were found to stimulate various cell types such as neurons, osteoblasts, and fibroblasts in both in vitro and in vivo studies. However, to our knowledge, no studies have been reported on the utility of CPs in stimulation of cancer or tumor cells in the literature. Here we report a facile fabrication method of self-doped sulfonated polyaniline (SPAN)-based interdigitated electrodes (IDEs) for controlled electrical stimulation of human osteosarcoma (HOS) cells. Increased degree of sulfonation was found to increase the SPAN conductivity, which in turn improved the cell attachment and cell growth without electrical stimulation. However, an enhanced cell growth was observed under controlled electrical (AC) stimulation at low applied voltage and frequency (≤800 mV and ≤1 kHz). The cell growth reached a maximum threshold at an applied voltage or frequency and beyond which pronounced cell death was observed. We believe that these organic electrodes may find utility in electrical stimulation of cancer or tumor cells for therapy and research and may also provide an alternative to the conventional metal-based electrodes.
导电聚合物 (CPs) 已被发现可在体外和体内研究中刺激各种细胞类型，如神经元、成骨细胞和成纤维细胞。然而，据我们所知，文献中尚无关于 CPs 在刺激癌细胞或肿瘤细胞方面的应用的研究。在这里，我们报告了一种简便的自掺杂磺化聚苯胺 (SPAN) 基叉指电极 (IDE) 的制备方法，用于对人骨肉瘤 (HOS) 细胞进行受控电刺激。研究发现，磺化程度的增加会提高 SPAN 的电导率，从而在没有电刺激的情况下改善细胞附着和细胞生长。然而，在低外加电压和频率 (≤800 mV 和 ≤1 kHz) 下进行受控电 (AC) 刺激时，观察到细胞生长增强。细胞生长在施加电压或频率达到最大值时达到最大值，超过该值时会观察到明显的细胞死亡。我们相信这些有机电极可用于癌症或肿瘤细胞的电刺激治疗和研究，并可能为传统的金属基电极提供替代方案。
