Kedney Mollie G, Strunk Kevin B, Giaquinto Lisa M, Wagner Jennifer A, Pollack Sidney, Patton Walter A
Department of Chemistry, Lebanon Valley College, Annville, Pennsylvania 17003-1400.
Biochem Mol Biol Educ. 2007 Nov;35(6):425-33. doi: 10.1002/bmb.105.
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS or simply MALDI)1 has become ubiquitous in the identification and analysis of biomacromolecules. As a technique that allows for the molecular weight determination of otherwise nonvolatile molecules, MALDI has had a profound impact in the molecular biosciences, especially in the field of proteomics. MALDI analysis of whole organisms allows for the generation of a phenotypic profile or "molecular fingerprint;" once established, these fingerprints can be used for identification purposes.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS或简称为MALDI）1在生物大分子的鉴定和分析中已无处不在。作为一种能够测定其他非挥发性分子分子量的技术，MALDI在分子生物科学领域，尤其是蛋白质组学领域产生了深远影响。对整个生物体进行MALDI分析能够生成表型图谱或“分子指纹”；一旦建立，这些指纹可用于鉴定目的。
