School of Medical Technology, Xuzhou Medical University, Xuzhou, 221004, China.
Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xian Jiaotong University Health Science Center, Xian, 710061, China.
Eur J Clin Microbiol Infect Dis. 2018 Jun;37(6):1021-1037. doi: 10.1007/s10096-018-3230-x. Epub 2018 Mar 22.
As strain variation and drug resistance become more pervasive, the prevention and control of infection have been a serious problem in recent years. The detection of pathogen is one of the most important parts of the process of diagnosis. Having a series of advantages, such as rapid response, high sensitivity, ease of use, and low cost, biosensors have received much attention and been studied deeply. Moreover, relying on its characteristics of small size, real time, and multiple analyses, biosensors have developed rapidly and used widely and are expected to be applied for microbiological detection in order to meet higher accuracy required by clinical diagnosis. The main goal of this contribution is not to simply collect and list all papers related to pathogen detection based on biosensors published recently, but to discuss critically the development and application of many kinds of biosensors such as electrochemical (amperometric, impedimetric, potentiometric, and conductometric), optical (fluorescent, fibre optic and surface plasmon resonance), and piezoelectric (quartz crystal microbalances and atomic force microscopy) biosensors in pathogen detection as well as the comparisons with the existing clinical detection methods (traditional culture, enzyme-linked immunosorbent assay, polymerase chain reaction, and mass spectrometry).
近年来，由于菌株变异和耐药性的日益普遍，感染的预防和控制已成为一个严重的问题。病原体的检测是诊断过程中最重要的环节之一。生物传感器具有快速响应、高灵敏度、易于使用和低成本等一系列优点，因此受到了广泛关注和深入研究。此外，生物传感器还凭借其体积小、实时、可进行多种分析等特点得到了快速发展和广泛应用，有望应用于微生物检测，以满足临床诊断所需的更高精度。本研究的主要目的不是简单地收集和列出最近基于生物传感器的病原体检测相关文献，而是批判性地讨论电化学（安培、阻抗、电位和电导）、光学（荧光、光纤和表面等离子体共振）和压电（石英晶体微天平和原子力显微镜）生物传感器在病原体检测中的发展和应用，以及与现有临床检测方法（传统培养、酶联免疫吸附测定、聚合酶链反应和质谱）的比较。
