Haag A M, Taylor S N, Johnston K H, Cole R B
Department of Chemistry, University of New Orleans, Louisiana 70148, USA.
J Mass Spectrom. 1998 Aug;33(8):750-6. doi: 10.1002/(SICI)1096-9888(199808)33:8<750::AID-JMS680>3.0.CO;2-1.
Several species of the genus Haemophilus are well known etiological agents of pneumonia, meningitis, conjunctivitis, epiglottitis and chancroid. However, identification and speciation of Haemophilus is both time consuming and labor intensive. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS) has been used by several investigators to profile proteins from intact and disrupted bacteria; consequently, MALDI/TOF-MS has emerged as a powerful tool in diagnostic bacteriology. This paper reports the use of MALDI/TOF-MS as a technique for the rapid identification and speciation of Haemophilus. This technique was used to not only identify the pathogen, H. ducreyi, but also to determine strain differences from different isolates. Mass spectral 'fingerprints' were obtained which permitted the rapid speciation of not only pathogenic forms of Haemophilus, but also those bacteria which are normally regarded as non-pathogenic and members of the normal flora. MALDI/TOF mass spectra can be acquired in 10 min, allowing the identification of Haemophilus spp. within 24 h rather than the 48 h or more needed for traditional bacteriological methods. In addition, these are the first mass spectral fingerprints available in the literature for many of these organisms.
嗜血杆菌属的几个菌种是肺炎、脑膜炎、结膜炎、会厌炎和软下疳的著名病原体。然而，嗜血杆菌的鉴定和分类既耗时又费力。多位研究人员已使用基质辅助激光解吸/电离飞行时间质谱（MALDI/TOF-MS）对完整和破碎细菌的蛋白质进行分析；因此，MALDI/TOF-MS已成为诊断细菌学中的一种强大工具。本文报道了使用MALDI/TOF-MS作为快速鉴定和分类嗜血杆菌的技术。该技术不仅用于鉴定病原体杜克雷嗜血杆菌，还用于确定不同分离株之间的菌株差异。获得了质谱“指纹图谱”，这不仅能快速对致病性嗜血杆菌进行分类，还能对通常被视为非致病性的细菌以及正常菌群的成员进行分类。MALDI/TOF质谱可在10分钟内获取，从而能在24小时内鉴定嗜血杆菌属菌种，而传统细菌学方法则需要48小时或更长时间。此外，这些是文献中首次报道的许多此类微生物的质谱指纹图谱。
