幽门螺杆菌编码糖基转移酶的两个同源基因在美国和日本的流行情况。
Prevalence of two homologous genes encoding glycosyltransferases of Helicobacter pylori in the United States and Japan.
机构信息
Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu-City, Oita, Japan.
出版信息
J Gastroenterol Hepatol. 2011 Sep;26(9):1451-6. doi: 10.1111/j.1440-1746.2011.06779.x.
BACKGROUND AND AIM
jhp0562 and β-(1,3)galT (jhp0563) of Helicobacter pylori have been suggested as novel virulent factors; however, the clinical associations and functions of these genes remain unclear. We examined the prevalence of jhp0562, β-(1,3)galT, and cagA in the United States (US) and Japanese populations.
METHODS
A total of 308 strains (171 from the US and 137 from Japan) were examined for the status of jhp0562, β-(1,3)galT, and cagA by polymerase chain reaction.
RESULTS
There were significant differences in the status of jhp0562, β-(1,3)galT and cagA between the US and Japanese populations (P < 0.001). In the US, the prevalence of β-(1,3)galT was significantly lower in strains isolated from patients with duodenal ulcer (DU) or gastric ulcer (GU) than those with gastritis (47.8% and 32.1% vs 72.0%, P < 0.01), and the absence of β-(1,3)galT was an independent factor discriminating DU and GU from gastritis (adjusted odds ratios, 4.21 and 8.52; 95% confidence intervals, 1.75 to 10.12 and 2.76 to 26.33, respectively). In the US, the prevalence of the jhp0562-positive/β-(1,3)galT-negative genotype was significantly higher in strains from DU and GU patients than in those from gastritis patients (50.0%, 67.9%, and 24.4%, P < 0.01) and the cagA status was significantly correlated with that of jhp0562 and inversely correlated with that of β-(1,3)galT. In contrast, the prevalence of these three genes was not significantly different in Japan.
CONCLUSIONS
jhp0562 or β-(1,3)galT can be used to discriminate peptic ulcers from gastritis in the US, but not in Japan.
背景与目的
幽门螺杆菌的 jhp0562 和 β-(1,3)galT（jhp0563）被认为是新的毒力因子，但这些基因的临床相关性和功能仍不清楚。我们在美国（US）和日本人群中检测了 jhp0562、β-(1,3)galT 和 cagA 的流行情况。
方法
通过聚合酶链反应检测了 308 株（美国 171 株，日本 137 株）菌株的 jhp0562、β-(1,3)galT 和 cagA 状态。
结果
美国和日本人群中 jhp0562、β-(1,3)galT 和 cagA 的状态存在显著差异（P<0.001）。在美国，十二指肠溃疡（DU）或胃溃疡（GU）患者分离株中 β-(1,3)galT 的发生率明显低于胃炎患者（47.8%和 32.1%比 72.0%，P<0.01），β-(1,3)galT 的缺失是 DU 和 GU 与胃炎区分的独立因素（调整优势比，4.21 和 8.52；95%置信区间，1.75 至 10.12 和 2.76 至 26.33）。在美国，DU 和 GU 患者分离株的 jhp0562 阳性/β-(1,3)galT 阴性基因型的发生率明显高于胃炎患者（50.0%、67.9%和 24.4%，P<0.01），cagA 状态与 jhp0562 显著相关，与 β-(1,3)galT 呈负相关。相比之下，在日本，这三种基因的流行率没有显著差异。
结论
在美国，jhp0562 或 β-(1,3)galT 可用于区分消化性溃疡和胃炎，但在日本不行。
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