Fleminger G, Hadas E, Wolf T, Solomon B
Department of Biotechnology, Faculty of Life Sciences, Tel-Aviv University, Israel.
Appl Biochem Biotechnol. 1990 Feb;23(2):123-37. doi: 10.1007/BF02798382.
Amino and hydrazyno derivatives of Eupergit C were prepared by reaction of the beads with hexamethylene diamine (HMD) and adipic acid dihydrazide (ADH), respectively. Monoclonal antibodies (mAbs) against carboxypeptidase A (CPA) and horse radish peroxidase (HRP) were prepared, and those that did not inhibit the respective enzymatic activities were selected. The carbohydrate moieties of these antibodies were oxidized by reaction with sodium periodate and then coupled onto the modified beads. The oxidation and coupling reactions were optimized to achieve highly active matrix-conjugated antibodies. Thus, antibody-matrix conjugates that possessed antigen-binding activities close to the theoretical value of 2 mol antigen bound/mol immobilized antibody were obtained.
通过将Eupergit C微珠分别与六亚甲基二胺（HMD）和己二酸二酰肼（ADH）反应，制备了Eupergit C的氨基和肼基衍生物。制备了抗羧肽酶A（CPA）和辣根过氧化物酶（HRP）的单克隆抗体（mAb），并选择了那些不抑制各自酶活性的抗体。这些抗体的碳水化合物部分通过与高碘酸钠反应进行氧化，然后偶联到修饰的微珠上。对氧化和偶联反应进行了优化，以获得高活性的基质偶联抗体。因此，获得了具有接近2 mol抗原结合/mol固定化抗体理论值的抗原结合活性的抗体-基质偶联物。
