Thayer School of Engineering, 8000 Cummings Hall, Dartmouth College, Hanover, New Hampshire 03755, USA.
Opt Lett. 2011 May 15;36(10):1911-3. doi: 10.1364/OL.36.001911.
A dark-field geometry spectral imaging system is presented to raster scan thick tissue samples in situ in 1.5 cm square sections, recovering full spectra from each 100 μm diameter pixel. This spot size provides adequate resolution for wide field scanning, while also facilitating scatter imaging without requiring sophisticated light-tissue transport modeling. The system is demonstrated showing accurate estimation of localized scatter parameters and the potential to recover absorption-based contrast from broadband reflectance data measured from 480 nm up to 750 nm in tissue phantoms. Results obtained from xenograft pancreas tumors show the ability to quantitatively image changes in localized scatter response in this fast-imaging geometry. The polychromatic raster scan design allows the rapid scanning necessary for use in surgical/clinical applications where timely decisions are required about tissue pathology.
提出了一种暗场几何光谱成像系统，用于原位对 1.5 厘米见方的厚组织样本进行光栅扫描，从每个 100μm 直径的像素中恢复全光谱。这种光斑尺寸为广角扫描提供了足够的分辨率，同时也便于进行散射成像，而无需复杂的光-组织传输建模。该系统通过演示显示了对局部散射参数的准确估计，并有可能从组织模拟体中测量的 480nm 至 750nm 的宽带反射率数据中恢复基于吸收的对比度。从异种移植物胰腺肿瘤中获得的结果表明，在这种快速成像几何中，能够定量成像局部散射响应的变化。多色光栅扫描设计允许快速扫描，这对于需要及时做出关于组织病理学决策的手术/临床应用非常重要。
