De Stasio P R, Taylor M W
Department of Biology, Indiana University, Bloomington 47405.
J Virol. 1990 Jun;64(6):2588-93. doi: 10.1128/JVI.64.6.2588-2593.1990.
Human recombinant gamma interferon and to a lesser extent alpha interferon were shown to inhibit herpes simplex virus type 1 replication in the human cell lines WISH and HEp-2. Dot blot analysis of viral DNA synthesis and viral RNA transcription indicated that the inhibition occurred at an early step in infection. A study of the early events after herpes simplex virus type 1 infection indicated that adsorption, penetration, uncoating, and transport of viral DNA were not affected by interferon. Northern (RNA) blot analysis revealed that both immediate-early and delayed-early gene transcription was inhibited by interferon. Transactivation of the immediate-early responsive element linked to a reporter gene (CAT or tk) was specifically inhibited by both classes of interferon. Our data would indicate that either the transactivating protein VP16 or the complex formed between VP16 and a host protein(s) is attenuated by interferon.
已证明，人重组γ干扰素以及程度较轻的α干扰素可抑制1型单纯疱疹病毒在人细胞系WISH和HEp-2中的复制。对病毒DNA合成和病毒RNA转录的斑点印迹分析表明，这种抑制作用发生在感染的早期阶段。一项关于1型单纯疱疹病毒感染后早期事件的研究表明，病毒DNA的吸附、穿透、脱壳及转运不受干扰素影响。Northern（RNA）印迹分析显示，立即早期和延迟早期基因转录均受到干扰素抑制。与报告基因（CAT或tk）相连的立即早期反应元件的反式激活受到两类干扰素的特异性抑制。我们的数据表明，反式激活蛋白VP16或VP16与一种或多种宿主蛋白形成的复合物被干扰素减弱。
