De Stasio P R, Taylor M W
Department of Biology, Indiana University, Bloomington, IN 47405.
Biochem Biophys Res Commun. 1989 Mar 15;159(2):439-44. doi: 10.1016/0006-291x(89)90011-9.
The effect of human recombinant interferons (IFNs) alpha and gamma on the transcription of Herpes Simplex virus type-1 (HSV-1) immediate early (IE) genes has been studied. Human amniotic cells (WISH) were transfected with a chimaeric plasmid containing the regulatory region of the HSV-1 IE genes controlling the expression of a reporter gene (CAT). Subsequently cells were infected with live HSV-1 to trans-activate the IE sequences. When cell samples were treated with IFN alpha or IFN gamma prior to viral infection, transcription of the CAT gene was greatly inhibited.
已经研究了人重组干扰素α和γ对单纯疱疹病毒1型（HSV-1）立即早期（IE）基因转录的影响。用人羊膜细胞（WISH）转染一种嵌合质粒，该质粒含有控制报告基因（CAT）表达的HSV-1 IE基因的调控区。随后用活的HSV-1感染细胞以反式激活IE序列。当在病毒感染前用干扰素α或干扰素γ处理细胞样品时，CAT基因的转录被大大抑制。
