Palermo-Dilts D A, Broker T R, Chow L T
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, New York 14642.
J Virol. 1990 Jun;64(6):3144-9. doi: 10.1128/JVI.64.6.3144-3149.1990.
Human papillomavirus type 1 (HPV-1) causes plantar warts. On the basis of previously mapped mRNAs and sequence homologies of HPV-1 to other papillomaviruses, we designed oligonucleotide primers and employed the polymerase chain reaction to recover HPV-1 cDNAs from plantar warts. Seven spliced RNA species were characterized, including three not previously detected, and the coding potentials of each were deduced. The most abundant viral mRNA encodes an E1i--E4 protein. One new species is predicted to encode the full-length E2 protein, and another can, theoretically, encode the E2-C or E1-M proteins, three products that regulate mRNA transcription and DNA replication. One RNA species originating from a novel HPV promoter in the upstream regulatory region has the potential to encode the minor capsid protein L2. A newly recognized E5a open reading frame (ORF) is contained in all mRNAs that are polyadenylated at the E-region poly(A) site and also in a putative L2 mRNA. Three distinct species, two of which are derived from the upstream regulatory region promoter, have the potential to encode the L1 protein; the third species also contains the entire coding region of the E1i--E4 protein 5' to the L1 ORF. Both the E1i--E4 mRNA and the potentially bicistronic L1 mRNA are derived from a promoter located in the E7 ORF. We uncovered no evidence of alternatively spliced mRNAs that could account for the multiple, abundant E4 proteins in plantar warts, suggesting that posttranslational modification is mainly responsible for the observed protein heterogeneity.
1型人乳头瘤病毒（HPV-1）可引起跖疣。基于先前绘制的HPV-1 mRNA图谱以及HPV-1与其他乳头瘤病毒的序列同源性，我们设计了寡核苷酸引物，并利用聚合酶链反应从跖疣中获取HPV-1 cDNA。我们对7种剪接RNA种类进行了表征，其中包括3种先前未检测到的，并且推导了每种的编码潜能。最丰富的病毒mRNA编码一种E1i-E4蛋白。预测一种新的RNA种类编码全长E2蛋白，另一种理论上可编码E2-C或E1-M蛋白，这三种产物可调节mRNA转录和DNA复制。一种源自上游调控区新型HPV启动子的RNA种类有可能编码次要衣壳蛋白L2。在所有在E区多聚腺苷酸化位点进行多聚腺苷酸化的mRNA以及一种假定的L2 mRNA中都包含一个新识别的E5a开放阅读框（ORF）。三种不同的RNA种类有可能编码L1蛋白，其中两种源自上游调控区启动子；第三种RNA种类在L1 ORF的5'端还包含E1i-E4蛋白的完整编码区。E1i-E4 mRNA和潜在的双顺反子L1 mRNA均源自位于E7 ORF中的启动子。我们没有发现可解释跖疣中多种丰富E4蛋白的可变剪接mRNA的证据，这表明翻译后修饰是观察到的蛋白质异质性的主要原因。
