Canadian Research Institute for Food Safety, University of Guelph, ON; N1G 2W1, Canada.
Virol J. 2011 May 19;8:242. doi: 10.1186/1743-422X-8-242.
Lytic bacteriophages have been applied successfully to control the growth of various foodborne pathogens. Sequencing of their genomes is considered as an important preliminary step to ensure their safety prior to food applications.
The lytic bacteriophage, ΦSboM-AG3, targets the important foodborne pathogen, Shigella. It is morphologically similar to phage ViI of Salmonella enterica serovar Typhi and a series of phages of Acinetobacter calcoaceticus and Rhizobium meliloti. The complete genome of ΦSboM-AG3 was determined to be 158 kb and was terminally redundant and circularly permuted. Two hundred and sixteen open reading frames (ORFs) were identified and annotated, most of which displayed homology to proteins of Salmonella phage ViI. The genome also included four genes specifying tRNAs.
This is the first time that a Vi-specific phage for Shigella has been described. There is no evidence for the presence of virulence and lysogeny-associated genes. In conclusion, the genome analysis of ΦSboM-AG3 indicates that this phage can be safely used for biocontrol purposes.
溶菌噬菌体已成功应用于控制各种食源性致病菌的生长。对其基因组进行测序被认为是在将其应用于食品之前确保其安全性的重要初步步骤。
溶菌噬菌体 ΦSboM-AG3 靶向重要的食源性致病菌志贺氏菌。它在形态上与沙门氏菌血清型 Typhi 的噬菌体 ViI 以及一系列不动杆菌和根瘤菌的噬菌体相似。ΦSboM-AG3 的完整基因组被确定为 158 kb，是末端冗余和环状循环的。鉴定并注释了 216 个开放阅读框（ORFs），其中大多数与沙门氏菌噬菌体 ViI 的蛋白质具有同源性。基因组还包括四个编码 tRNA 的基因。
这是首次描述针对志贺氏菌的特异性 Vi 噬菌体。没有发现与毒力和溶原性相关基因的存在。综上所述，对 ΦSboM-AG3 的基因组分析表明，该噬菌体可安全用于生物防治目的。
