Wilm Matthias
University College Dublin - Conway Institute, Ireland.
Mol Cell Proteomics. 2011 May 19. doi: 10.1074/mcp.R111.009407.
Electrospray ionization is today the most widely used ionization technique in chemical and bio-chemical analysis. Interfaced with a mass spectrometer it allows to investigate the molecular composition of liquid samples. With electrospray a large variety of chemical substances can be ionized. There is no limitation in mass which enables even the investigation of large non-covalent protein complexes. Its high ionization efficiency profoundly changed bio-molecular sciences because proteins can be identified and quantified on trace amounts in a high throughput fashion. This review article focusses mainly on the exploration of the underlying ionization mechanism. Some ionization characteristics are discussed which are related to this mechanism. Typical spectra of peptides, proteins and non-covalent complexes are shown and the quantitative character of spectra is highlighted. Finally the possibilities and limitations in measuring the association constant of bivalent non-covalent complexes are described.
如今，电喷雾电离是化学和生化分析中使用最广泛的电离技术。与质谱仪联用，它能够研究液体样品的分子组成。通过电喷雾，可以使多种化学物质发生电离。在质量方面没有限制，甚至能够对大型非共价蛋白质复合物进行研究。其高电离效率深刻地改变了生物分子科学，因为可以以高通量方式对痕量蛋白质进行鉴定和定量。这篇综述文章主要聚焦于对潜在电离机制的探索。讨论了一些与此机制相关的电离特性。展示了肽、蛋白质和非共价复合物的典型光谱，并强调了光谱的定量特性。最后描述了测量二价非共价复合物缔合常数的可能性和局限性。
