Department of Animal and Poultry Science, Centre for Genetic Improvement of Livestock, University of Guelph, ON N1G 2W1, Canada.
Mamm Genome. 2011 Oct;22(9-10):583-8. doi: 10.1007/s00335-011-9332-8. Epub 2011 May 20.
Mycobacterium avium ssp. paratuberculosis (MAP) infection causes a chronic granulomatous inflammatory condition of the bovine gut that is characterized by diarrhea, progressive weight loss, and emaciation, and ultimately leads to loss in productivity and profitability of dairy operations. The host cytokine machinery is known to play an important role in protecting against MAP infection. Therefore, the goal of the present study was to assess whether polymorphisms in candidate genes encoding important cytokines and cytokine receptors are associated with MAP infection status of dairy cattle. MAP infection status was evaluated based on serum and milk enzyme-linked immunosorbent assays (ELISAs) for MAP-specific antibodies. Twenty previously reported polymorphisms in genes encoding bovine interferon gamma (IFNG), IFNGR1, IFNGR2, IL22, IL22RA1, IL12RB1, IL12RB2, and IL23R were genotyped in a resource population of 446 dairy Holsteins with known MAP infection status, and logistic regression was used to assess the statistical association with a binomial MAP infection status phenotype. Four SNPs in IFNGR2, IL12RB1, IL12RB2, and IL23R were found to be associated with the MAP infection status of the resource population. These results underscore the importance of cytokines and their receptors in conferring protection against MAP infection and warrant further functional characterization of these associations.
鸟分枝杆菌亚种副结核（MAP）感染会导致牛肠道的慢性肉芽肿性炎症，其特征是腹泻、进行性体重减轻和消瘦，并最终导致奶制品生产和盈利能力下降。宿主细胞因子机制被认为在对抗 MAP 感染中起着重要作用。因此，本研究的目的是评估编码重要细胞因子和细胞因子受体的候选基因中的多态性是否与奶牛的 MAP 感染状态相关。MAP 感染状态是基于针对 MAP 特异性抗体的血清和牛奶酶联免疫吸附测定（ELISA）进行评估的。在具有已知 MAP 感染状态的 446 头荷斯坦奶牛资源群体中，对编码牛干扰素γ（IFNG）、IFNGR1、IFNGR2、IL22、IL22RA1、IL12RB1、IL12RB2 和 IL23R 的 20 个先前报道的多态性进行了基因分型，并使用逻辑回归评估了与二项式 MAP 感染状态表型的统计学关联。IFNGR2、IL12RB1、IL12RB2 和 IL23R 中的 4 个 SNP 与资源群体的 MAP 感染状态相关。这些结果强调了细胞因子及其受体在赋予对抗 MAP 感染的保护中的重要性，并需要进一步对这些关联进行功能表征。
