Centre for Genetic Improvement of Livestock, Department of Animal and Poultry Science, University of Guelph, Guelph, ON, Canada.
Anim Genet. 2011 Aug;42(4):354-60. doi: 10.1111/j.1365-2052.2010.02153.x. Epub 2011 Feb 23.
Mycobacterium avium ssp. paratuberculosis (MAP) causes a chronic, granulomatous inflammatory condition of the intestines in ruminants and wild-type species. It causes significant economic losses to the dairy and beef industries owing to reduced productivity, premature culling and mortality. Bovine peptidoglycan recognition protein 1 is an important pattern recognition molecule that is capable of directly killing microorganisms. The goal of this study was to identify single nucleotide polymorphisms (SNPs) in the gene encoding bovine peptidoglycan recognition protein 1 and to assess their association with susceptibility to MAP infection in dairy cattle. Blood and milk samples were collected from Holsteins in Southwestern and Eastern Ontario and tested for MAP infection using blood and milk ELISAs. A resource population consisting of 197 infected (S/P > 0.25) and 242 healthy (S/P < 0.10) cattle was constructed. Sequencing of pooled DNA was used to identify three SNPs (c.102G>C, c.480G>A and c.625C>A) that were genotyped in the resource population. Statistical analysis was performed using a logistic regression model fitting the additive and dominance effects of each SNP in the model. SNP c.480G>A (P = 0.054) was found to be associated with susceptibility to MAP infection. Cows with a copy of the major allele 'G' at this locus had an odds ratio of 1.51 (95% CI: 0.99-2.31) for being infected with MAP.
鸟分枝杆菌副结核亚种（MAP）引起反刍动物和野生型动物的慢性肉芽肿性肠炎。由于生产力下降、提前淘汰和死亡，它给奶牛和肉牛产业造成了巨大的经济损失。牛肽聚糖识别蛋白 1 是一种重要的模式识别分子，能够直接杀死微生物。本研究的目的是鉴定编码牛肽聚糖识别蛋白 1 的基因中的单核苷酸多态性（SNP），并评估其与奶牛对 MAP 感染的易感性的关系。从安大略省西南部和东部的荷斯坦奶牛中采集血液和牛奶样本，并用血液和牛奶 ELISA 检测 MAP 感染情况。构建了一个包含 197 头感染（S/P > 0.25）和 242 头健康（S/P < 0.10）牛的资源群体。对混合 DNA 的测序用于鉴定三个 SNP（c.102G>C、c.480G>A 和 c.625C>A），并在资源群体中进行了基因分型。使用逻辑回归模型拟合模型中每个 SNP 的加性和显性效应，对统计分析进行了拟合。SNP c.480G>A（P = 0.054）与 MAP 感染易感性相关。在该基因座中具有主要等位基因“G”拷贝的奶牛感染 MAP 的优势比为 1.51（95%CI：0.99-2.31）。
