Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamilnadu 608 002, India.
Toxicol In Vitro. 2011 Oct;25(7):1366-75. doi: 10.1016/j.tiv.2011.05.007. Epub 2011 May 12.
Radiotherapy may be effectively combined with plant derived radiosensitizers. Ferulic acid, a naturally occurring phenolic acid, has been reported to have free radical producing properties. In the present study, the radiosensitisation potential of ferulic acid has been tested in two cervical cancer cell lines (HeLa and ME-180) in vitro. Percentage of growth inhibition (MTT assay), colony survival, levels of lipid peroxidation (TBARS, CD and LHP), antioxidant status (SOD, CAT, GPx and GSH), oxidative DNA damage (% tail DNA, tail length, tail moment and Olive tail moment), apoptotic morphological changes (AO/EtBr staining) and intracellular ROS levels (DCFH-DA) were estimated. The present results show that ferulic acid (FA) enhances radiation effects by increasing lipid peroxidative markers in HeLa and ME-180 cells. We observed significant enhancement of ROS levels during ferulic acid plus radiation treatment. FA treatment alone increased intracellular ROS levels indicate its prooxidant nature. Similarly, we observed enhanced oxidative DNA damage and apoptotic morphological changes in FA plus radiation treated cells. The present data suggest radiation sensitizing property of FA in cervical cancer cells. Further investigations warrants to substantiate the present findings.
放射治疗可以与植物来源的放射增敏剂有效结合。阿魏酸是一种天然存在的酚酸，已被报道具有产生自由基的特性。在本研究中，阿魏酸的放射增敏作用在体外的两种宫颈癌细胞系（HeLa 和 ME-180）中进行了测试。采用生长抑制百分比（MTT 测定法）、集落存活、脂质过氧化水平（TBARS、CD 和 LHP）、抗氧化状态（SOD、CAT、GPx 和 GSH）、氧化 DNA 损伤（%尾 DNA、尾长、尾矩和 Olive 尾矩）、凋亡形态变化（AO/EtBr 染色）和细胞内 ROS 水平（DCFH-DA）进行了评估。目前的结果表明，阿魏酸（FA）通过增加 HeLa 和 ME-180 细胞中的脂质过氧化标记物来增强辐射效应。我们观察到在阿魏酸加辐射处理期间 ROS 水平显著增加。FA 单独处理可增加细胞内 ROS 水平，表明其具有促氧化特性。同样，我们观察到在 FA 加辐射处理的细胞中氧化 DNA 损伤和凋亡形态变化增强。本研究数据表明 FA 在宫颈癌细胞中具有放射增敏作用。需要进一步的研究来证实目前的发现。
