Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, People's Republic of China.
Acta Biomater. 2011 Aug;7(8):3070-7. doi: 10.1016/j.actbio.2011.05.005. Epub 2011 May 9.
Carbon nanotube (CNT)-organic polymer hybrids have important potential applications in the immobilization of therapeutic biomolecules. Recently developed CNT-organic polymer composites require the use of organic solvents for their preparation and have limited polymer functionalization. To address these limitations, multi-walled CNT (MWCNT)-chitosan nanoparticle (CS NP) hybrids have been synthesized in situ by an ionotropic gelation process, which is extremely mild and involves the mixture of two aqueous solutions at room temperature. The MWCNT-CS NP hybrids were characterized by atomic force microscopy and thermogravimetric analysis. Under optimal conditions the CS NP can be tethered to the MWCNT surface in high density and with relatively uniform coverage. The MWCNT-CS NP hybrids show good dispersibility and stability in aqueous solutions. In order to evaluate the potential utilization of the hybrids as protein carries the cytotoxicity to HeLa cells and protein immobilization (of bovine serum albumin (BSA), used here as a model) capacity of the hybrids were investigated in detail. The results demonstrate that the MWCNT-CS NP hybrids are biocompatible at concentrations up to 100 μg mL(-1) for 24 h incubation. The MWCNT-CS NP hybrids improve the BSA immobilization efficiency 0.8 times and simultaneously decrease the cellular toxicity by about 50% compared with carboxylated MWCNT.
碳纳米管（CNT）-有机聚合物杂化物在固定治疗生物分子方面具有重要的潜在应用。最近开发的 CNT-有机聚合物复合材料在制备过程中需要使用有机溶剂，并且对聚合物的功能化有限。为了解决这些限制，通过离子凝胶化过程原位合成了多壁 CNT（MWCNT）-壳聚糖纳米颗粒（CS NP）杂化物，该过程非常温和，涉及两种水溶液在室温下混合。通过原子力显微镜和热重分析对 MWCNT-CS NP 杂化物进行了表征。在最佳条件下，CS NP 可以高密度地键合到 MWCNT 表面，并且具有相对均匀的覆盖度。MWCNT-CS NP 杂化物在水溶液中具有良好的分散性和稳定性。为了评估该杂化物作为蛋白质载体的潜在用途，详细研究了该杂化物对 HeLa 细胞的细胞毒性和蛋白质固定（牛血清白蛋白（BSA），此处用作模型）能力。结果表明，MWCNT-CS NP 杂化物在 24 h 孵育时的浓度高达 100 μg mL（-1）时具有生物相容性。与羧基化 MWCNT 相比，MWCNT-CS NP 杂化物提高了 BSA 固定化效率 0.8 倍，同时降低了约 50％的细胞毒性。
