Tan Julia Meihua, Saifullah Bullo, Kura Aminu Umar, Fakurazi Sharida, Hussein Mohd Zobir
Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Laboratory of Vaccine and Immunotherapeutics, Institute of Bioscience (IBS), Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
Nanomaterials (Basel). 2018 May 31;8(6):389. doi: 10.3390/nano8060389.
Four drug delivery systems were formulated by non-covalent functionalization of carboxylated single walled carbon nanotubes using biocompatible polymers as coating agent (i.e., Tween 20, Tween 80, chitosan or polyethylene glycol) for the delivery of levodopa, a drug used in Parkinson's disease. The chemical interaction between the coating agent and carbon nanotubes-levodopa conjugate was confirmed by Fourier transform infrared (FTIR) and Raman studies. The drug release profiles were revealed to be dependent upon the type of applied coating material and this could be further adjusted to a desired rate to meet different biomedical conditions. In vitro drug release experiments measured using UV-Vis spectrometry demonstrated that the coated conjugates yielded a more prolonged and sustained release pattern compared to the uncoated conjugate. Cytotoxicity of the formulated conjugates was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using normal mouse embryonic fibroblast 3T3 cell line. Compared to the non-coated conjugate, the MTT data indicated that the coating procedure improved the biocompatibility of all systems by 34⁻41% when the concentration used exceeded 100 μg/mL. In conclusion, the comprehensive results of this study suggest that carbon nanotubes-based drug carrier coated with a suitable biomaterial may possibly be a potential nanoparticle system that could facilitate drug delivery to the brain with tunable physicochemical properties.
通过使用生物相容性聚合物（即吐温20、吐温80、壳聚糖或聚乙二醇）作为包衣剂对羧基化单壁碳纳米管进行非共价功能化，制备了四种药物递送系统，用于递送帕金森病治疗药物左旋多巴。通过傅里叶变换红外光谱（FTIR）和拉曼光谱研究证实了包衣剂与碳纳米管-左旋多巴缀合物之间的化学相互作用。药物释放曲线显示取决于所应用的包衣材料类型，并且可以进一步调整至所需速率以满足不同的生物医学条件。使用紫外可见光谱法进行的体外药物释放实验表明，与未包衣的缀合物相比，包衣的缀合物产生了更持久和持续的释放模式。使用正常小鼠胚胎成纤维细胞3T3细胞系，通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法研究了所制备缀合物的细胞毒性。与未包衣的缀合物相比，MTT数据表明，当使用的浓度超过100μg/mL时，包衣过程使所有系统的生物相容性提高了34%-41%。总之，本研究的综合结果表明，用合适的生物材料包衣的基于碳纳米管的药物载体可能是一种潜在的纳米颗粒系统，可促进药物向大脑的递送，并具有可调节的物理化学性质。
