Key Laboratory of Modern Agriculture Equipment and Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, China.
Anal Chim Acta. 2011 Jun 10;695(1-2):84-8. doi: 10.1016/j.aca.2011.03.042. Epub 2011 Apr 14.
This work reports a rapid and sensitive organophosphates (OPs) amperometric biosensor based on acetylcholinesterase (AChE) immobilized on CdS-decorated graphene (CdS-G) nanocomposite. The as-prepared biosensor shows high affinity to acetylthiocholine (ATCl) with a Michaelis-Menten constant (K(m)) value of 0.24 mM. A rapid inhibition time (2 min) is obtained due to the integration of the CdS-G nanocomposite. Based on the inhibition of OPs on the enzymatic activity of the immobilized AChE, and used carbaryl as the model compound, the resulting biosensor exhibits excellent performance for OPs detection including good reproducibility, acceptable stability, and a reliable linear relationship between the inhibition and log[carbaryl] from 2 ng mL⁻¹ up to 2 μg mL⁻¹ with a detection limit of 0.7 ng mL⁻¹,which provides a new promising tool for analysis of enzyme inhibitors.
这项工作报道了一种基于 CdS 修饰的石墨烯 (CdS-G) 纳米复合材料固定化乙酰胆碱酯酶 (AChE) 的快速灵敏的有机磷 (OPs) 电流型生物传感器。所制备的生物传感器对乙酰硫代胆碱 (ATCl) 具有高亲和力，米氏常数 (K(m)) 值为 0.24 mM。由于 CdS-G 纳米复合材料的集成，获得了快速的抑制时间 (2 分钟)。基于 OPs 对固定化 AChE 酶活性的抑制作用，并以carbaryl 作为模型化合物，所得生物传感器在 OPs 检测方面表现出优异的性能，包括良好的重现性、可接受的稳定性，以及抑制与 log[carbaryl]之间从 2ng/mL 到 2μg/mL 的可靠线性关系，检测限为 0.7ng/mL，为分析酶抑制剂提供了一种有前途的新工具。
