Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok, Thailand.
Fitoterapia. 2011 Sep;82(6):798-804. doi: 10.1016/j.fitote.2011.04.013. Epub 2011 May 9.
The ethanolic extract from stems of a Thai medicinal plant, Alstonia macrophylla Wall. ex G. Don (Apocynaceae) showed a significant inhibitory effect on acetylcholinesterase (AChE) determined by using Ellman assay. Four compounds i.e., a bisindole alkaloid, macralstonine (1), a new bisindole alkaloid, thungfaine (2), a secoiridoid glycoside, sweroside (3) and a new secoiridoid glycoside, naresuanoside (4) were isolated. Compound 4 showed moderate AChE and butyrylcholinesterase (BChE) inhibitory effects. Interestingly, compound 4 inhibited cell growth on human androgen-sensitive prostate cancer cell line (LNCaP) but no effect on viability of human foreskin fibroblast cells (HF).
泰国药用植物大驳骨（夹竹桃科）茎的乙醇提取物在埃勒曼试验中对乙酰胆碱酯酶（AChE）表现出显著的抑制作用。从该植物中共分离得到了 4 种化合物，即双吲哚生物碱马卡罗汀（1）、新的双吲哚生物碱图恩法因（2）、环烯醚萜苷物西罗莫司（3）和新的环烯醚萜苷物奈瑞索苷（4）。化合物 4 对乙酰胆碱酯酶和丁酰胆碱酯酶均表现出中等抑制作用。有趣的是，化合物 4 抑制人雄激素敏感前列腺癌细胞系（LNCaP）的细胞生长，但对人包皮成纤维细胞（HF）的活力没有影响。
