Tan Maria Carmen S, Carranza Mary Stephanie S, Linis Virgilio C, Malabed Raymond S, Oyong Glenn G
Chemistry Department, Biology Department, and Molecular Science Unit Laboratory Center for Natural Sciences and Environmental Research, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines.
Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.
ACS Omega. 2019 May 30;4(5):9488-9496. doi: 10.1021/acsomega.9b00082. eCollection 2019 May 31.
The objective of this research was to find the possible pharmacognosy of the bark of the Philippine Wall. ex G.Don (AM). Gas chromatographic-mass spectral (GC-EI-MS) characterization and energy dispersive X-ray spectroscopy (EDX) were performed to detect the bioactive constituents. EDX analysis of AM bark displayed a high content of potassium (3.26%) and calcium (2.96%). Eight constituents were detected in AM crude dichloromethane (DCM) extracts, which consisted of a long-chain unsaturated fatty acid (17:0) and fatty acid esters such as ethyl hexadecanoate and methyl hexadecanoate. Extraction of AM bark using methanol and dimethyl sulfoxide (MeOH/DMSO) solvents resulted in the identification of 17 constituents, principally alkaloids (alstonerine, 34.38%; strictamin, 5.23%; rauvomitin, 4.29%; and brucine, 3.66%) and triterpenoids (γ-sitosterol, 3.85%; lupeol, 3.00%; 24-methylenecycloartanol, 2.81%; campesterol, 2.71%; β-amyrin, 2.30%; and stigmasterol, 2.13%). MeOH/DMSO samples of AM were used in the selected bioassays. The samples exhibited efficient free radical scavenging activity (IC = 0.71 mg/mL) and were noncytotoxic to normal HDFn (IC > 100 μg/mL) and neoplastic THP-1 cell lines (IC = 67.22 μg/mL) while highly degenerative to MCF-7 (IC = 6.34 μg/mL), H69PR (IC = 7.05 μg/mL), and HT-29 (IC = 9.10 μg/mL). Most interestingly, the AM samples inhibited the northern Philippine Cobra's ( Taylor) venom (IC = 297.27 ± 9.33 μg/mL) through a secretory phospholipase A assay.
本研究的目的是探寻菲律宾Wall. ex G.Don（AM）树皮可能的生药学特性。采用气相色谱 - 质谱联用（GC - EI - MS）表征和能量色散X射线光谱法（EDX）检测生物活性成分。AM树皮的EDX分析显示钾含量较高（3.26%）和钙含量较高（2.96%）。在AM二氯甲烷（DCM）粗提物中检测到8种成分，包括一种长链不饱和脂肪酸（17:0）以及脂肪酸酯，如十六烷酸乙酯和十六烷酸甲酯。使用甲醇和二甲基亚砜（MeOH/DMSO）溶剂提取AM树皮，鉴定出17种成分，主要是生物碱（阿枯米定碱，34.38%；士的宁，5.23%；萝芙木亭，4.29%；马钱子碱，3.66%）和三萜类化合物（γ - 谷甾醇，3.85%；羽扇豆醇，3.00%；24 - 亚甲基环阿尔廷醇，2.81%；菜油甾醇，2.71%；β - 香树脂醇，2.30%；豆甾醇，2.13%）。AM的MeOH/DMSO样品用于选定的生物测定。这些样品表现出高效的自由基清除活性（IC = 0.71 mg/mL），对正常的人皮肤成纤维细胞（HDFn，IC > 100 μg/mL）和肿瘤性THP - 1细胞系无细胞毒性（IC = 67.22 μg/mL），而对MCF - 7（IC = 6.34 μg/mL）、H69PR（IC = 7.05 μg/mL）和HT - 29（IC = 9.10 μg/mL）具有高度降解作用。最有趣的是，通过分泌型磷脂酶A测定，AM样品抑制了菲律宾北部眼镜蛇（泰勒）的毒液（IC = 297.27 ± 9.33 μg/mL）。
