Chen Y S, Vaughn J M
Department of Applied Science, Brookhaven National Laboratory, Upton, New York 11973.
Appl Environ Microbiol. 1990 May;56(5):1363-6. doi: 10.1128/aem.56.5.1363-1366.1990.
The inactivation of single-particle stocks of human (type 2, Wa) and simian (SA-11) rotaviruses by chlorine dioxide was investigated. Experiments were conducted at 4 degrees C in a standard phosphate-carbonate buffer. Both virus types were rapidly inactivated, within 20 s under alkaline conditions, when chlorine dioxide concentrations ranging from 0.05 to 0.2 mg/liter were used. Similar reductions of 10(5)-fold in infectivity required additional exposure time of 120 s at 0.2 mg/liter for Wa and at 0.5 mg/liter for SA-11, respectively, at pH 6.0. The inactivation of both virus types was moderate at neutral pH, and the sensitivities to chlorine dioxide were similar. The observed enhancement of virucidal efficiency with increasing pH was contrary to earlier findings with chlorine- and ozone-treated rotavirus particles, where efficiencies decreased with increasing alkalinity. Comparison of 99.9% virus inactivation times revealed ozone to be the most effective virucidal agent among these three disinfectants.
研究了二氧化氯对人（2型，Wa株）和猴（SA - 11株）轮状病毒单颗粒毒株的灭活作用。实验在4℃的标准磷酸盐 - 碳酸盐缓冲液中进行。当使用浓度范围为0.05至0.2毫克/升的二氧化氯时，两种病毒类型在碱性条件下20秒内迅速灭活。在pH 6.0时，对于Wa株，在0.2毫克/升浓度下需要额外120秒的暴露时间才能使感染性类似地降低10^5倍；对于SA - 11株，在0.5毫克/升浓度下需要额外120秒的暴露时间。两种病毒类型在中性pH下的灭活作用中等，对二氧化氯的敏感性相似。观察到随着pH升高病毒灭活效率增强，这与早期用氯和臭氧处理轮状病毒颗粒的研究结果相反，早期研究中随着碱度增加效率降低。99.9%病毒灭活时间的比较表明，臭氧是这三种消毒剂中最有效的杀病毒剂。
