发酵乳杆菌对产气荚膜梭菌β2 毒素产生的影响。
Effect of Lactobacillus fermentum on beta2 toxin production by Clostridium perfringens.
机构信息
Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, P.O. Box 80.158, 3508TD Utrecht, The Netherlands.
出版信息
Appl Environ Microbiol. 2011 Jul;77(13):4406-11. doi: 10.1128/AEM.03002-10. Epub 2011 May 20.
Abstract
Clostridium perfringens, although a member of the normal gut flora, is also an important cause of intestinal disease in animals and, to a lesser extent, in humans. Disease is associated with the production of one or more toxins, and little is known about environmental influences on the production of these toxins. One of the health-promoting effects of lactic acid bacteria (LAB) is the establishment and maintenance of a low pH in the intestine since an acidic environment inhibits the growth of many potentially harmful bacteria. Here, the effect of the LAB Lactobacillus fermentum on beta2 toxin production by C. perfringens is described. Coculturing of C. perfringens with L. fermentum showed that under in vitro conditions, L. fermentum was capable of silencing beta2 toxin production by C. perfringens without influencing bacterial viability. The reduction in toxin production was shown to be most likely a result of the decline in pH. Quantitative PCR showed that the reduction in beta2 toxin production was due to a decrease in cpb2 mRNA. These results suggest that in the intestine, the production of beta2 toxin by C. perfringens might be regulated by other members of the normal intestinal flora.
摘要
产气荚膜梭菌虽然是正常肠道菌群的成员，但也是动物肠道疾病的重要病因，在一定程度上也是人类肠道疾病的病因。该病与一种或多种毒素的产生有关，但人们对这些毒素产生的环境影响知之甚少。乳酸菌（LAB）的一个健康促进作用是在肠道中建立和维持低 pH 值，因为酸性环境会抑制许多潜在有害细菌的生长。本文描述了 LAB 发酵乳杆菌对产气荚膜梭菌β2 毒素产生的影响。体外共培养实验表明，在体外条件下，发酵乳杆菌能够沉默产气荚膜梭菌β2 毒素的产生，而不影响细菌活力。产毒减少很可能是由于 pH 值下降所致。定量 PCR 显示，β2 毒素产生的减少是由于 cpb2mRNA 的减少所致。这些结果表明，在肠道中，产气荚膜梭菌β2 毒素的产生可能受到正常肠道菌群其他成员的调节。
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