益生菌枯草芽孢杆菌对A型产气荚膜梭菌在Caco-2细胞培养中细胞毒性的影响。
The effects of probiotic Bacillus subtilis on the cytotoxicity of Clostridium perfringens type a in Caco-2 cell culture.
作者信息
Poormontaseri Maryam, Hosseinzadeh Saeid, Shekarforoush Seyed Shahram, Kalantari Tahereh
机构信息
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, P.O. Box: 71441-69155, Shiraz, Iran.
Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.
出版信息
BMC Microbiol. 2017 Jul 4;17(1):150. doi: 10.1186/s12866-017-1051-1.
BACKGROUND
Some Bacillus strains have recently been identified for potential use as probiotics and food additives. The present study evaluated the antimicrobial effects of Bacillus subtilis ATCC 6633 and its metabolite on the enterotoxin and vegetative cells, spore and germinated spore of Clostridium perfringens type A in Caco-2 cells.
RESULTS
We used flow cytometry and MTT assays to evaluate the cytotoxicity effect of treatments. According to the results, the most cell survival was found in the 4% crude antimicrobial substance (CAS) with the vegetative form of C. perfringens among co-cultured groups. Furthermore, the apoptosis and necrosis in co-cultured groups were significantly decreased (P < 0.05).
CONCLUSION
The present results suggested the crucial role of the current probiotic in the control of various forms of C. perfringens type A which was investigated for the first time. Also, the majority of treatments showed higher cell viability in flow cytometry compared to the MTT assay.
背景
最近已鉴定出一些芽孢杆菌菌株有作为益生菌和食品添加剂的潜在用途。本研究评估了枯草芽孢杆菌ATCC 6633及其代谢产物对A型产气荚膜梭菌肠毒素、营养细胞、芽孢和萌发芽孢在Caco-2细胞中的抗菌作用。
结果
我们使用流式细胞术和MTT试验来评估处理的细胞毒性作用。根据结果，在共培养组中，4%的粗抗菌物质（CAS）与A型产气荚膜梭菌营养形式共同培养时细胞存活率最高。此外，共培养组中的凋亡和坏死显著减少（P < 0.05）。
结论
目前的结果表明这种益生菌在控制各种形式的A型产气荚膜梭菌中起关键作用，这是首次进行此类研究。此外，与MTT试验相比，大多数处理在流式细胞术中显示出更高的细胞活力。
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