Department of Medical Biochemistry, Wroclaw Medical University, Chałubińskiego 10, PL 50-368 Wrocław, Poland.
Pharmacol Rep. 2011;63(2):501-17. doi: 10.1016/s1734-1140(11)70517-5.
Nitrogranulogen (NTG) may modify the character of inflammatory reactions. These modifications are a result of cytotoxic and mutagenic effects. NTG has high affinity to DNA and causes disorders in the synthesis of acute phase proteins (e.g., haptoglobin, transferrin, fibrinogen, and complement protein C3). Our previous studies have shown that small doses of NTG can enhance immunological defense reactions in the organism. The aim of the current studies was to determine how different NTG doses cause changes in the values of biochemical parameters in pleuritis-induced rats. The animals were randomized into five groups: Group I - control group; Group II - IP (induced pleuritis) group; Group III - NTG5 group; Group IV - NTG50 group; Group V - NTG600 group. Blood was collected from all groups of animals at 24, 48, and 72 h after the initiation of the carrageenin-induced inflammatory reaction. These investigations revealed that a dose of 5 μg NTG/kg b.w. (body weight) can change the character of the inflammation. Our studies also show that a dose of 600 μg NTG/kg b.w. causes a rapid decrease in the level of C3 at the 72 h of the experiment (after 3 applications every 24 h), which indicates a cytotoxic action of such a large NTG dose. NTG used at doses of 50 and 600 μg/kg b.w. causes the opposite metabolism of albumins and other serum proteins. Our studies show that the different doses of NTG have distinct effects on the inflammatory reaction.
硝普钠（NTG）可能会改变炎症反应的特征。这些变化是细胞毒性和致突变作用的结果。NTG 与 DNA 具有高亲和力，并导致急性期蛋白（例如触珠蛋白、转铁蛋白、纤维蛋白原和补体蛋白 C3）的合成紊乱。我们之前的研究表明，小剂量的 NTG 可以增强机体的免疫防御反应。本研究的目的是确定不同剂量的 NTG 如何引起胸膜炎诱导大鼠生化参数值的变化。将动物随机分为五组：I 组 - 对照组；II 组 - IP（诱导胸膜炎）组；III 组 - NTG5 组；IV 组 - NTG50 组；V 组 - NTG600 组。在角叉菜胶诱导的炎症反应开始后 24、48 和 72 小时，从所有组别的动物中采集血液。这些研究表明，5μg NTG/kg b.w.（体重）的剂量可以改变炎症的特征。我们的研究还表明，600μg NTG/kg b.w. 的剂量在实验的 72 小时（在每 24 小时应用 3 次后）会迅速降低 C3 水平，这表明如此大剂量的 NTG 具有细胞毒性作用。50 和 600μg/kg b.w. 的 NTG 剂量会导致白蛋白和其他血清蛋白的代谢相反。我们的研究表明，不同剂量的 NTG 对炎症反应有不同的影响。
