Całkosiński Ireneusz, Rosińczuk-Tonderys Joanna, Bronowicka-Szydełko Agnieszka, Dzierzba Katarzyna, Bazan Justyna, Dobrzyński Maciej, Majda Jacek, Gamian Andrzej
Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Poland
Department of Nervous System Diseases, The Faculty of Health Science, Wroclaw Medical University, Poland.
Toxicol Ind Health. 2015 Jun;31(6):510-22. doi: 10.1177/0748233713475497. Epub 2013 Feb 13.
The aim of this study was to evaluate the effect of tocopherol on pleuritis-induced rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Rats were treated with a single TCDD dose of 5 μg/kg body weight (b.w.) and then for 3 weeks they were daily supplemented with tocopherol at a dose of 30 mg/kg b.w. The inflammation was initiated by intrapleural injection of a single dose of 1% carrageenin solution in a volume of 0.15 ml. Changes in biochemical blood parameters were measured three times at the 24th, 72nd and 120th hour of pleuritis and the blood was collected from 20 animals of each group of rats (group with the control inflammation; group treated with TCDD and with control inflammation; group treated with TCDD, supplemented with tocopherol and with the inflammation). The following biochemical parameters were measured: tumor necrosis factor (TNF), interleukin-1 (IL-1), IL-2, IL-4, IL-6, procollagen, telopeptide, fibrinogen, cholesterol, urea, creatinine, aspartate aminotransferase (AspAT) and alanine aminotransferase (AlAT). Daily supplementation of tocopherol caused significant changes in the level of TNF, IL-1, IL-4, IL-6, urea, creatinine, AspAT and AlAT. According to the results of these studies, we suggest that tocopherol supplementation in high doses could act as a protective treatment to improve liver metabolism.
本研究的目的是评估生育酚对暴露于2,3,7,8-四氯二苯并对二恶英（TCDD）的胸膜炎诱导大鼠的影响。大鼠接受单次5μg/kg体重（b.w.）的TCDD剂量治疗，然后连续3周每天补充30mg/kg b.w.的生育酚。通过胸腔内注射0.15ml单剂量1%角叉菜胶溶液引发炎症。在胸膜炎的第24、72和120小时对每组20只大鼠（对照组炎症组；TCDD治疗且有对照组炎症组；TCDD治疗、补充生育酚且有炎症组）的血液生化参数进行三次测量。测量了以下生化参数：肿瘤坏死因子（TNF）、白细胞介素-1（IL-1）、IL-2、IL-4、IL-6、前胶原、端肽、纤维蛋白原、胆固醇、尿素、肌酐、天冬氨酸转氨酶（AspAT）和丙氨酸转氨酶（AlAT）。每天补充生育酚导致TNF、IL-1、IL-4、IL-6、尿素、肌酐、AspAT和AlAT水平发生显著变化。根据这些研究结果，我们建议高剂量补充生育酚可作为一种保护性治疗，以改善肝脏代谢。
