Ghose T, Lee C L, Fernandez L A, Lee S H, Raman R, Colp P
Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada.
Cancer Res. 1990 Jun 15;50(12):3737-42.
In order to investigate the association between various karyotypes of human tumor cells and biological behavior of tumors such as tumorigenicity, rate of growth, and the capacity to form metastasis, six chromosomally distinctive clones were isolated from an Epstein-Barr virus-transformed human chronic lymphocytic leukemic B-cell line which progressively grew and metastasized in irradiated nude mice. When inoculated into nude mice one clone (D10-1) with the karyotype of 46,XY,dup(1)(q11----q32) was more tumorigenic, grew faster, and produced more metastases than the other five clones. When mixtures of different clone-derived cells were grown in vitro or inoculated s.c. into nude mice the proportion of D10-1 cells was higher than their expected numbers in the cultures, s.c. tumors, and splenic and lymph nodal metastases. The growth and metastatic potential of the D10-1 clone were inhibited when cells from this clone were mixed with one or more clones that had slower growth. Duplication of 1q has been observed as a secondary aberration in human hematological malignancies and solid cancers. Our results demonstrate that duplication of the chromosome segment of 1q11----1q32 is associated with advantages in proliferation and metastasis formation.
为了研究人类肿瘤细胞的各种核型与肿瘤生物学行为（如致瘤性、生长速率和形成转移的能力）之间的关联，从一株爱泼斯坦 - 巴尔病毒转化的人类慢性淋巴细胞白血病B细胞系中分离出六个染色体特征不同的克隆，该细胞系在受辐照的裸鼠中逐渐生长并发生转移。当接种到裸鼠体内时，一个核型为46,XY,dup(1)(q11----q32)的克隆（D10 - 1）比其他五个克隆具有更强的致瘤性、生长更快且产生更多转移灶。当不同克隆来源的细胞混合物在体外培养或皮下接种到裸鼠体内时，D10 - 1细胞在培养物、皮下肿瘤以及脾脏和淋巴结转移灶中的比例高于预期数量。当将D10 - 1克隆的细胞与一个或多个生长较慢的克隆混合时，D10 - 1克隆的生长和转移潜能受到抑制。1q重复已在人类血液系统恶性肿瘤和实体癌中作为继发性畸变被观察到。我们的结果表明，1q11----1q32染色体片段的重复与增殖和转移形成方面的优势相关。
