Immune Modulator Research Center, Korea Research Institute of Bioscience and Biotechnology, 685-4 Yangchung-ri, Ochang-eup, Cheongwon-gun, Chungbuk, Republic of Korea.
Inflammation. 2012 Apr;35(2):535-44. doi: 10.1007/s10753-011-9343-3.
Elaeocarpus petiolatus is known to exert active oxygen scavenging, anti-aging, and whitening actions. However, the biological effects of E. petiolatus on inflammation and the underlying mechanisms are yet to be established. In the present study, we investigated the anti-inflammatory effects of the ethanol extract from E. petiolatus (EPE) bark in murine Raw264.7 macrophages stimulated with lipopolysaccharide (LPS). EPE inhibited the production of PGE(2), TNF-α, and IL-1β in a dose-dependent manner in Raw264.7 cells stimulated with LPS. The decrease in PGE(2) production was correlated with reduced COX-2 expression. Furthermore, EPE suppressed the phosphorylation of extracellular signal-related kinases (ERK), c-Jun N-terminal kinase (JNK), and p38 as well as translocation of the NF-κB p65 subunit from the cytosol to nucleus. Our results suggest that EPE exerts anti-inflammatory activity through inhibition of inflammatory mediators, such as PGE(2), TNF-α, and IL-1β, and downregulation of COX-2 via suppression of NF-κB translocation and phosphorylation of ERK, JNK, and p38 in LPS-stimulated Raw264.7 cells.
油甘子被认为具有清除活性氧、抗衰老和美白作用。然而，油甘子对炎症的生物学效应及其潜在机制尚不清楚。在本研究中，我们研究了油甘子树皮乙醇提取物（EPE）对脂多糖（LPS）刺激的小鼠 Raw264.7 巨噬细胞的抗炎作用。EPE 以剂量依赖的方式抑制 LPS 刺激的 Raw264.7 细胞中 PGE（2）、TNF-α 和 IL-1β 的产生。PGE（2）产生的减少与 COX-2 表达的降低相关。此外，EPE 抑制了细胞外信号相关激酶（ERK）、c-Jun N 末端激酶（JNK）和 p38 的磷酸化以及 NF-κB p65 亚基从细胞质向细胞核的易位。我们的结果表明，EPE 通过抑制炎症介质（如 PGE（2）、TNF-α 和 IL-1β）的产生，以及通过抑制 NF-κB 易位和 ERK、JNK 和 p38 的磷酸化来下调 COX-2，从而发挥抗炎活性在 LPS 刺激的 Raw264.7 细胞中。
