Park Sang-Yong, Kim Ho-Bin, Kim Jeong-Hoon, Lee Joo-Mi, Kim Sang-Rae, Shin Heon-Sub, Yi Tae-Hoo
Department of Oriental Medicinal Materials & Processing, Kyung Hee University, Gyeonggi 446-701, Korea.
Woongjin Food Co., Ltd., Seoul 100-705, Korea.
Prev Nutr Food Sci. 2014 Jan;19(1):10-8. doi: 10.3746/pnf.2014.19.1.010.
In this study, Woongjin fermented red ginseng extract (WFRG) was evaluated for its potential ability to act as an adjuvant for the immune response of mice. For the in vitro study, macrophages were treated with serial concentrations (1 μg/mL, 10 μg/mL, and 100 μg/mL) of WFRG. For in vivo studies, mice were administered different concentrations (10 mg/kg/day, 100 mg/kg/day, and 200 mg/kg/day) of WFRG orally for 21 days. In vitro, the production of nitric oxide and TNF-α by RAW 264.7 cells increased in a dose-dependent manner. In vivo, WFRG enhanced the proliferation of splenocytes induced by two mitogens (i.e., concanavalin A and lipopolysaccharide [LPS]) and increased LPS-induced production of TNF-α and IL-6, but not IL-1β. In conclusion, WFRG has the potential to modulate immune function and should be further investigated as an immunostimulatory agent.
在本研究中，对熊津发酵红参提取物（WFRG）作为小鼠免疫反应佐剂的潜在能力进行了评估。在体外研究中，用系列浓度（1μg/mL、10μg/mL和100μg/mL）的WFRG处理巨噬细胞。在体内研究中，给小鼠口服不同浓度（10mg/kg/天、100mg/kg/天和200mg/kg/天）的WFRG，持续21天。在体外，RAW 264.7细胞产生一氧化氮和TNF-α的量呈剂量依赖性增加。在体内，WFRG增强了两种促有丝分裂原（即伴刀豆球蛋白A和脂多糖[LPS]）诱导的脾细胞增殖，并增加了LPS诱导的TNF-α和IL-6的产生，但未增加IL-1β的产生。总之，WFRG具有调节免疫功能的潜力，应作为一种免疫刺激剂进行进一步研究。
