老年人视网膜毛细血管基底膜的改变。
Basement membrane changes in capillaries of the ageing human retina.
机构信息
MacTel Laboratory Research Group, UCL Institute of Ophthalmology, University College London, London, UK.
出版信息
Br J Ophthalmol. 2011 Sep;95(9):1316-22. doi: 10.1136/bjo.2011.204222. Epub 2011 May 23.
OBJECTIVES
The ultrastructural appearance of retinal capillaries can yield important information about disease mechanisms, but is not well characterised in human post mortem samples. We therefore aimed to create a baseline for the appearance of capillaries and establish how this is influenced by post mortem fixation delays and donor age.
METHODS
Electron microscopy was used to characterise retinal capillaries in 20 anonymous donors (with no known eye diseases) of various ages and with various post mortem fixation delays. In addition, samples from six patients with conditions that are known to affect the retinal vasculature (four cases of type 2 diabetes without diabetic retinopathy, one case of diabetic retinopathy and one case of macular telangiectasia type 2) were analysed.
RESULTS
Vacuoles were found in capillary basement membranes at the vessel-glia interface in all samples, from both the normal and disease cases. Vacuole frequency increased with donor age but was not influenced by post mortem fixation delays.
CONCLUSION
Vacuoles in the basement membrane are a normal feature of adult human retinal capillaries and do not indicate disease. Their incidence increases with age and might be a contributing factor to late-onset pathologies of the retinal vasculature.
目的
视网膜毛细血管的超微结构外观可以提供有关疾病机制的重要信息，但在人体死后样本中尚未得到很好的描述。因此，我们旨在为毛细血管的外观建立一个基线，并确定死后固定延迟和供体年龄如何影响这一点。
方法
使用电子显微镜对来自 20 位匿名供体（无已知眼部疾病）的不同年龄和不同死后固定延迟的视网膜毛细血管进行特征描述。此外，还分析了来自 6 名已知会影响视网膜血管的疾病患者的样本（4 例 2 型糖尿病无糖尿病视网膜病变、1 例糖尿病视网膜病变和 1 例 2 型黄斑部毛细血管扩张症）。
结果
在所有正常和疾病样本的血管-神经胶质界面的毛细血管基底膜中都发现有空泡。空泡频率随供体年龄的增加而增加，但不受死后固定延迟的影响。
结论
基底膜中的空泡是成年人大鼠视网膜毛细血管的正常特征，并不表明存在疾病。它们的发生率随年龄的增加而增加，可能是视网膜血管晚期发病机制的一个促成因素。
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