Project Directorate on Poultry, Rajendranagar, Hyderabad, 500030, Andhra Pradesh, India.
Vet Res Commun. 2011 Jun;35(5):321-7. doi: 10.1007/s11259-011-9472-z. Epub 2011 May 24.
Toll-like receptor (TLR) family is one of the important members of innate immune system that recognizes conserved microbial patterns and induces innate immune response. They also act as a link to adaptive immune response. Nitric oxide (NO) is a multi-functional mediator with diverse physiological and immunological roles. In the present study TLR mRNA expression in heterophils, serum nitric oxide level and iNOS (inducible Nitric Oxide Synthase) gene polymorphism were investigated in cockerels of two Indian native chicken breeds, Aseel and Kadaknath. TLR (4 and 5) mRNA expression as quantified by real time RT-PCR revealed Kadaknath males expressed significantly (P < 0.01) higher TLR4 mRNA than Aseel males. iNOS gene polymorphism analyzed by PCR-RFLP method revealed difference in allele frequency. Kadaknath males had higher allele B frequency (0.81) than Aseel males (0.56). However, there were no genotype and breed effect on serum nitric oxide level. Based on the present study we conclude that Kadaknath has comparatively higher innate immunity levels than Aseel, however further investigations are needed.
Toll 样受体 (TLR) 家族是先天免疫系统的重要成员之一，能够识别保守的微生物模式并诱导先天免疫反应。它们还充当与适应性免疫反应的联系。一氧化氮 (NO) 是一种多功能介质，具有多种生理和免疫学作用。在本研究中，研究了两种印度本土鸡品种（Aseel 和 Kadaknath）公鸡中性粒细胞中的 TLR mRNA 表达、血清一氧化氮水平和 iNOS（诱导型一氧化氮合酶）基因多态性。通过实时 RT-PCR 定量 TLR（4 和 5）mRNA 表达显示，Kadaknath 公鸡的 TLR4 mRNA 表达明显高于 Aseel 公鸡（P<0.01）。通过 PCR-RFLP 方法分析 iNOS 基因多态性显示等位基因频率存在差异。Kadaknath 公鸡的等位基因 B 频率（0.81）高于 Aseel 公鸡（0.56）。然而，血清一氧化氮水平没有基因型和品种效应。根据本研究，我们得出结论，与 Aseel 相比，Kadaknath 具有相对较高的先天免疫水平，但需要进一步研究。
