Ki-67/MIB-1、mitosin、survivin、pHH3 和 DNA 拓扑异构酶 IIα 在人脑胶质母细胞瘤中的增殖标志物评估——免疫组化研究。
Evaluation of the proliferation markers Ki-67/MIB-1, mitosin, survivin, pHH3, and DNA topoisomerase IIα in human anaplastic astrocytomas--an immunohistochemical study.
机构信息
Department of Laboratory Medicine, Children's and Women's Health, Norwegian University of Science and Technology, Trondheim, Norway.
出版信息
Diagn Pathol. 2011 May 24;6:43. doi: 10.1186/1746-1596-6-43.
BACKGROUND
Histological malignancy grading of astrocytomas can be challenging despite criteria given by the World Health Organisation (WHO). Grading is fundamental for optimal prognostication and treatment, and additional biomarkers are needed to support the histopathological diagnosis. Estimation of proliferative activity has gained much enthusiasm, and the present study was designed to evaluate and compare novel immunohistochemical proliferative markers in human anaplastic astrocytomas.
METHODS
Proliferative activity was determined in twenty-seven cases with antibodies reactive against the Ki-67 antigen, mitosin, survivin, pHH3, and DNA topoisomerase IIα, and they were mutually compared as well as related to mitotic activity.
RESULTS
The markers correlated well with each other, but poorly with mitoses, probably because of small and squeezed tumour samples, in which identification of mitoses can be difficult. Positive association to overall survival was observed as well.
CONCLUSIONS
Our data show that these markers may assist significantly in the evaluation of proliferative activity in anaplastic astrocytomas and even have prognostic value.
背景
尽管世界卫生组织 (WHO) 给出了标准，但星形细胞瘤的组织学恶性程度分级仍然具有挑战性。分级对于最佳预后和治疗至关重要，需要额外的生物标志物来支持组织病理学诊断。增殖活性的评估得到了广泛关注，本研究旨在评估和比较人类间变性星形细胞瘤中新型免疫组织化学增殖标志物。
方法
用针对 Ki-67 抗原、mitosin、survivin、pHH3 和 DNA 拓扑异构酶 IIα 的抗体对 27 例病例进行了增殖活性测定，并对它们进行了相互比较，并与有丝分裂活性相关。
结果
这些标志物彼此之间相关性良好，但与有丝分裂相关性较差，这可能是因为肿瘤样本较小且被挤压，在这些样本中，有丝分裂的识别可能很困难。观察到与总生存期的正相关。
结论
我们的数据表明，这些标志物可能对评估间变性星形细胞瘤的增殖活性有很大帮助，甚至具有预后价值。
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