从多能干细胞中诱导产生新皮层兴奋性神经元。
Deriving excitatory neurons of the neocortex from pluripotent stem cells.
机构信息
Department of Neurology, University of California, San Francisco, CA 94143, USA.
出版信息
Neuron. 2011 May 26;70(4):645-60. doi: 10.1016/j.neuron.2011.05.006.
Abstract
The human cerebral cortex is an immensely complex structure that subserves critical functions that can be disrupted in developmental and degenerative disorders. Recent innovations in cellular reprogramming and differentiation techniques have provided new ways to study the cellular components of the cerebral cortex. Here, we discuss approaches to generate specific subtypes of excitatory cortical neurons from pluripotent stem cells. We review spatial and temporal aspects of cortical neuron specification that can guide efforts to produce excitatory neuron subtypes with increased resolution. Finally, we discuss distinguishing features of human cortical development and their translational ramifications for cortical stem cell technologies.
摘要
人类大脑皮层是一种极其复杂的结构，它为发育和退行性疾病所破坏的关键功能提供支持。细胞重编程和分化技术的最新创新为研究大脑皮层的细胞成分提供了新的方法。在这里，我们讨论了从多能干细胞产生特定类型兴奋性皮层神经元的方法。我们回顾了皮层神经元特化的空间和时间方面，这些方面可以指导产生具有更高分辨率的兴奋性神经元亚型的努力。最后，我们讨论了人类皮层发育的区别特征及其对皮层干细胞技术的转化意义。
相似文献
1
Deriving excitatory neurons of the neocortex from pluripotent stem cells.从多能干细胞中诱导产生新皮层兴奋性神经元。
Neuron. 2011 May 26;70(4):645-60. doi: 10.1016/j.neuron.2011.05.006.
2
Development and regeneration of projection neuron subtypes of the cerebral cortex.大脑皮层投射神经元亚型的发育和再生。
Sci Prog. 2010;93(Pt 2):151-69. doi: 10.3184/003685010X12705764469952.
3
Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses.人类大脑皮层从多能干细胞发育到功能性兴奋性突触。
Nat Neurosci. 2012 Feb 5;15(3):477-86, S1. doi: 10.1038/nn.3041.
4
Generation of cortical neurons from pluripotent stem cells.诱导多能干细胞分化为皮质神经元。
Prog Brain Res. 2012;201:183-95. doi: 10.1016/B978-0-444-59544-7.00009-3.
5
Maturation of AMPAR composition and the GABAAR reversal potential in hPSC-derived cortical neurons.人源诱导多能干细胞源性皮质神经元上 AMPAR 组成和 GABAAR 反转电位的成熟。
J Neurosci. 2014 Mar 12;34(11):4070-5. doi: 10.1523/JNEUROSCI.5410-13.2014.
6
Shaping our minds: stem and progenitor cell diversity in the mammalian neocortex.塑造我们的思维：哺乳动物新皮层中的干细胞和祖细胞多样性。
Neuron. 2013 Jan 9;77(1):19-34. doi: 10.1016/j.neuron.2012.12.022.
7
Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks.人多能干细胞向大脑皮层神经元和神经网络的定向分化。
Nat Protoc. 2012 Oct;7(10):1836-46. doi: 10.1038/nprot.2012.116. Epub 2012 Sep 13.
8
Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells.联合小分子抑制可加速从人多能干细胞中获得功能性皮质神经元。
Nat Biotechnol. 2017 Feb;35(2):154-163. doi: 10.1038/nbt.3777. Epub 2017 Jan 23.
9
Embryonic progenitor pools generate diversity in fine-scale excitatory cortical subnetworks.胚胎祖细胞库在精细尺度兴奋性皮质亚网络中产生多样性。
Nat Commun. 2019 Nov 19;10(1):5224. doi: 10.1038/s41467-019-13206-1.
10
Human pluripotent stem cell-derived radial glia recapitulate developmental events and provide real-time access to cortical neurons and astrocytes.人多能干细胞来源的放射状胶质细胞重现发育事件，并提供对皮质神经元和星形胶质细胞的实时观察途径。
Stem Cells Transl Med. 2015 May;4(5):437-47. doi: 10.5966/sctm.2014-0137. Epub 2015 Apr 1.
引用本文的文献
1
Two parallel lineage-committed progenitors contribute to the developing brain.两个平行的谱系定向祖细胞参与发育中的大脑形成。
bioRxiv. 2025 Jul 5:2025.07.02.662771. doi: 10.1101/2025.07.02.662771.
2
Stimulating Wnt signaling reveals context-dependent genetic effects on gene regulation in primary human neural progenitors.刺激Wnt信号通路揭示了对原代人神经祖细胞基因调控的背景依赖性遗传效应。
Nat Neurosci. 2024 Dec;27(12):2430-2442. doi: 10.1038/s41593-024-01773-6. Epub 2024 Sep 30.
3
Morphological and transcriptomic analyses of stem cell-derived cortical neurons reveal mechanisms underlying synaptic dysfunction in schizophrenia.基于干细胞的皮质神经元的形态学和转录组学分析揭示了精神分裂症中突触功能障碍的潜在机制。
Genome Med. 2023 Jul 28;15(1):58. doi: 10.1186/s13073-023-01203-5.
4
Structural neuroimaging phenotypes and associated molecular and genomic underpinnings in autism: a review.自闭症中的结构性神经影像表型及其相关的分子和基因组基础：综述
Front Neurosci. 2023 Jun 30;17:1172779. doi: 10.3389/fnins.2023.1172779. eCollection 2023.
5
Brain Organization and Human Diseases.脑的组织结构与人类疾病
Cells. 2022 May 14;11(10):1642. doi: 10.3390/cells11101642.
6
Signal requirement for cortical potential of transplantable human neuroepithelial stem cells.移植的人神经上皮干细胞的皮质电势的信号要求。
Nat Commun. 2022 May 23;13(1):2844. doi: 10.1038/s41467-022-29839-8.
7
Cerebral gray matter injuries in infants with intraventricular hemorrhage.脑室出血婴儿的脑灰质损伤。
Semin Perinatol. 2022 Aug;46(5):151595. doi: 10.1016/j.semperi.2022.151595. Epub 2022 Mar 12.
8
Uncovering specificity of endogenous TAU aggregation in a human iPSC-neuron TAU seeding model.在人诱导多能干细胞衍生神经元TAU种子模型中揭示内源性TAU聚集的特异性
iScience. 2021 Dec 18;25(1):103658. doi: 10.1016/j.isci.2021.103658. eCollection 2022 Jan 21.
9
Application of reprogrammed patient cells to investigate the etiology of neurological and psychiatric disorders.应用重编程的患者细胞来研究神经和精神疾病的病因。
Front Biol (Beijing). 2012 Jun;7(3):179-188. doi: 10.1007/s11515-012-1216-0. Epub 2012 Apr 1.
10
Screening Platforms for Genetic Epilepsies-Zebrafish, iPSC-Derived Neurons, and Organoids.遗传性癫痫的筛查平台——斑马鱼、iPSC 衍生神经元和类器官。
Neurotherapeutics. 2021 Jul;18(3):1478-1489. doi: 10.1007/s13311-021-01115-5. Epub 2021 Sep 30.
本文引用的文献
1
Modelling schizophrenia using human induced pluripotent stem cells.使用人类诱导多能干细胞来模拟精神分裂症。
Nature. 2011 May 12;473(7346):221-5. doi: 10.1038/nature09915. Epub 2011 Apr 13.
2
A new subtype of progenitor cell in the mouse embryonic neocortex.在小鼠胚胎新皮质中发现一种新的祖细胞亚型。
Nat Neurosci. 2011 May;14(5):555-61. doi: 10.1038/nn.2807. Epub 2011 Apr 10.
3
Oblique radial glial divisions in the developing mouse neocortex induce self-renewing progenitors outside the germinal zone that resemble primate outer subventricular zone progenitors.发育中的小鼠新皮层中的斜向放射状胶质分裂在生发区外诱导出自我更新的祖细胞，这些祖细胞类似于灵长类动物的外侧脑室下区祖细胞。
J Neurosci. 2011 Mar 9;31(10):3683-95. doi: 10.1523/JNEUROSCI.4773-10.2011.
4
LRRK2 mutant iPSC-derived DA neurons demonstrate increased susceptibility to oxidative stress.LRRK2 突变型 iPSC 衍生的 DA 神经元对氧化应激的敏感性增加。
Cell Stem Cell. 2011 Mar 4;8(3):267-80. doi: 10.1016/j.stem.2011.01.013.
5
Intrinsic transition of embryonic stem-cell differentiation into neural progenitors.胚胎干细胞分化为神经祖细胞的内在转变。
Nature. 2011 Feb 24;470(7335):503-9. doi: 10.1038/nature09726. Epub 2011 Feb 16.
6
Subregional specification of embryonic stem cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals.通过定时和组合处理外源性信号对胚胎干细胞衍生的腹侧端脑组织进行亚区划分。
J Neurosci. 2011 Feb 2;31(5):1919-33. doi: 10.1523/JNEUROSCI.5128-10.2011.
7
Small-molecule inhibitors of bone morphogenic protein and activin/nodal signals promote highly efficient neural induction from human pluripotent stem cells.小分子骨形态发生蛋白和激活素/诺达信号抑制剂促进高效的人多能干细胞神经诱导。
J Neurosci Res. 2011 Feb;89(2):117-26. doi: 10.1002/jnr.22547. Epub 2010 Dec 8.
8
A role for intermediate radial glia in the tangential expansion of the mammalian cerebral cortex.中间放射状胶质细胞在哺乳动物大脑皮层的切向扩张中的作用。
Cereb Cortex. 2011 Jul;21(7):1674-94. doi: 10.1093/cercor/bhq238. Epub 2010 Dec 2.
9
A model for neural development and treatment of Rett syndrome using human induced pluripotent stem cells.利用人类诱导多能干细胞进行神经发育和雷特综合征治疗的模型。
Cell. 2010 Nov 12;143(4):527-39. doi: 10.1016/j.cell.2010.10.016.
10
Cortical progenitor expansion, self-renewal and neurogenesis-a polarized perspective.皮层祖细胞的扩增、自我更新和神经发生——一个有极化观点的视角。
Curr Opin Neurobiol. 2011 Feb;21(1):23-35. doi: 10.1016/j.conb.2010.10.002. Epub 2010 Oct 29.
Zotero 插件