Centre de recherche de Gif, Institut de Chimie des Substances Naturelles, CNRS, avenue de la Terrasse, 91198 Gif-sur-Yvette, France.
Curr Opin Chem Biol. 2011 Oct;15(5):725-32. doi: 10.1016/j.cbpa.2011.04.017. Epub 2011 May 24.
Time-Of-Flight Secondary Ion Mass Spectrometry is compared to other mass spectrometry imaging techniques, and recent improvements of the experimental methods, driven by biological and biomedical applications, are described and discussed. This review shows that this method that can be considered as a micrometric molecular histology is particularly efficient for obtaining images of various lipid species at the surface of a tissue sample, without sample preparation, and with a routine spatial resolution of 1μm or less.
飞行时间二次离子质谱与其他质谱成像技术进行了比较，并描述和讨论了近年来生物和生物医学应用推动的实验方法的改进。该综述表明，这种可以被认为是微观分子组织学的方法对于在组织样品表面获得各种脂质物种的图像特别有效，无需样品制备，并且具有常规的 1μm 或更小的空间分辨率。
