高效直接扩展谱β-内酰胺酶检测的多重实时 PCR 法：利用有限的遗传标记准确进行表型分类。
Efficient direct extended-spectrum β-lactamase detection by multiplex real-time PCR: accurate assignment of phenotype by use of a limited set of genetic markers.
机构信息
CIDM, Level 3 ICPMR Building, Westmead Hospital, Westmead NSW 2145, Australia.
出版信息
J Clin Microbiol. 2011 Aug;49(8):3074-7. doi: 10.1128/JCM.02647-10. Epub 2011 May 25.
Abstract
The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum β-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae.
摘要
潜在基因的数量和多样性可能会使基因方法变得复杂，难以快速检测可传播的扩展谱β-内酰胺酶基因。我们开发了一种基于先前调查中确定的靶标的稳健的多重实时 PCR 检测方法，并使用该方法检测了 617 例连续的产扩展谱β-内酰胺酶（ESBL）的肠杆菌科临床分离株中的相关基因。
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