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高效直接扩展谱β-内酰胺酶检测的多重实时 PCR 法:利用有限的遗传标记准确进行表型分类。

Efficient direct extended-spectrum β-lactamase detection by multiplex real-time PCR: accurate assignment of phenotype by use of a limited set of genetic markers.

机构信息

CIDM, Level 3 ICPMR Building, Westmead Hospital, Westmead NSW 2145, Australia.

出版信息

J Clin Microbiol. 2011 Aug;49(8):3074-7. doi: 10.1128/JCM.02647-10. Epub 2011 May 25.

DOI:10.1128/JCM.02647-10
PMID:21613435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3147779/
Abstract

The number and diversity of genes potentially complicate genetic approaches to the rapid detection of transmissible extended-spectrum β-lactamase genes. We developed a robust multiplexed real-time PCR assay based on targets identified in a prior survey and used this to detect relevant genes in 617 consecutive clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae.

摘要

潜在基因的数量和多样性可能会使基因方法变得复杂,难以快速检测可传播的扩展谱β-内酰胺酶基因。我们开发了一种基于先前调查中确定的靶标的稳健的多重实时 PCR 检测方法,并使用该方法检测了 617 例连续的产扩展谱β-内酰胺酶(ESBL)的肠杆菌科临床分离株中的相关基因。

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本文引用的文献

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Antimicrob Agents Chemother. 2010 Nov;54(11):4914-6. doi: 10.1128/AAC.00878-10. Epub 2010 Sep 7.
2
New disturbing trend in antimicrobial resistance of gram-negative pathogens.革兰氏阴性病原体抗菌耐药性的新的令人不安的趋势。
PLoS Pathog. 2009 Mar;5(3):e1000221. doi: 10.1371/journal.ppat.1000221. Epub 2009 Mar 27.
3
Dominance of blaCTX-M within an Australian extended-spectrum beta-lactamase gene pool.blaCTX-M在澳大利亚超广谱β-内酰胺酶基因库中的优势地位。
Antimicrob Agents Chemother. 2008 Nov;52(11):4198-202. doi: 10.1128/AAC.00107-08. Epub 2008 Aug 25.
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bla(IMP-4) in different genetic contexts in Enterobacteriaceae isolates from Australia.澳大利亚肠杆菌科分离株中不同基因背景下的bla(IMP-4)
Antimicrob Agents Chemother. 2008 Aug;52(8):2984-7. doi: 10.1128/AAC.01634-07. Epub 2008 May 19.
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Extended-spectrum beta-lactamase-producing Enterobacteriaceae: an emerging public-health concern.产超广谱β-内酰胺酶肠杆菌科细菌:一个新出现的公共卫生问题。
Lancet Infect Dis. 2008 Mar;8(3):159-66. doi: 10.1016/S1473-3099(08)70041-0.
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Clinical and economic impact of common multidrug-resistant gram-negative bacilli.常见多重耐药革兰氏阴性杆菌的临床和经济影响
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