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TaqMan 多重 PCR 检测法的开发，用于检测质粒介导的 AmpC β-内酰胺酶基因。

Development of a TaqMan multiplex PCR assay for detection of plasmid-mediated ampC β-lactamase genes.

机构信息

Creighton University School of Medicine Department of Medical Microbiology and Immunology Center for Research in Anti-Infectives and Biotechnology, Omaha, Nebraska, USA.

出版信息

J Clin Microbiol. 2012 Nov;50(11):3722-5. doi: 10.1128/JCM.02038-12. Epub 2012 Aug 15.

DOI:10.1128/JCM.02038-12
PMID:22895035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3486237/
Abstract

A multiplex, real-time TaqMan assay was designed to identify clinical isolates carrying plasmid-mediated ampC genes. The specificity and sensitivity of this assay were 100% when testing characterized AmpC/non-AmpC-producing isolates and randomly selected clinical isolates. This is a rapid assay that can be performed in a clinical microbiology laboratory.

摘要

设计了一种多重实时 TaqMan 检测法，以鉴定携带质粒介导的 AmpC 基因的临床分离株。当检测特征性 AmpC/非 AmpC 产生菌和随机选择的临床分离株时，该检测法的特异性和灵敏度均为 100%。这是一种快速检测法，可在临床微生物学实验室中进行。

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