1,2,3,4,6-五没食子酰基-β-D-葡萄糖处理的 MDA-MB-231 乳腺癌细胞的全基因组表达谱：癌症代谢的分子靶点。
The genome-wide expression profile of 1,2,3,4,6-penta-O-galloyl-β-D-glucose-treated MDA-MB-231 breast cancer cells: molecular target on cancer metabolism.
机构信息
Cancer Preventive Material Development Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea.
出版信息
Mol Cells. 2011 Aug;32(2):123-32. doi: 10.1007/s10059-011-2254-1. Epub 2011 May 24.
Abstract
1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG), a polyphenolic compound isolated from Rhus chinensis Mill. PGG has been known to have anti-tumor, anti-angiogenic and anti-diabetic activities. The present study revealed another underlying molecular target of PGG in MDA-MB-231 breast cancer cells by using Illumina Human Ref-8 expression BeadChip assay. Through the Beadstudio v3 micro assay program to compare the identified genes expressed in PGG-treated MDA-MB-231 cells with untreated control, we found several unique genes that are closely associated with pyruvate metabolism, glycolysis/gluconeogenesis and tyrosine metabolism, including PC, ACSS2, ACACA, ACYP2, ALDH3B1, FBP1, PRMT2 and COMT. Consistent with microarray data, real-time RT-PCR confirmed the significant down-regulation of these genes at mRNA level in PGG-treated MDA-MB-231 cells. Our findings suggest the potential of PGG as anticancer agent for breast cancer cells by targeting cancer metabolism genes.
摘要
1,2,3,4,6-五没食子酰基-β-D-葡萄糖（PGG）是从盐肤木中分离得到的多酚化合物，已知具有抗肿瘤、抗血管生成和抗糖尿病活性。本研究通过 Illumina Human Ref-8 表达 BeadChip 分析，揭示了 PGG 在 MDA-MB-231 乳腺癌细胞中的另一个潜在分子靶点。通过 Beadstudio v3 微检测程序比较 PGG 处理的 MDA-MB-231 细胞与未处理对照细胞中表达的鉴定基因，我们发现了几个与丙酮酸代谢、糖酵解/糖异生和酪氨酸代谢密切相关的独特基因，包括 PC、ACSS2、ACACA、ACYP2、ALDH3B1、FBP1、PRMT2 和 COMT。与微阵列数据一致，实时 RT-PCR 证实 PGG 处理的 MDA-MB-231 细胞中这些基因在 mRNA 水平的显著下调。我们的研究结果表明，PGG 通过靶向癌症代谢基因，有可能成为乳腺癌细胞的抗癌药物。
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