传染性胃肠炎病毒在几种源自猪肾的细胞系中的增殖以及胰蛋白酶对病毒生长的影响。

The multiplication of transmissible gastroenteritis viruses in several cell lines originated from porcine kidney and effects of trypsin on the growth of the viruses.

作者信息

Honda E, Takahashi H, Okazaki K, Minetoma T, Kumagai T

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.

出版信息

Nihon Juigaku Zasshi. 1990 Apr;52(2):217-24. doi: 10.1292/jvms1939.52.217.

DOI:10.1292/jvms1939.52.217

PMID:2161476

Abstract

Plaque formation, replication and related cytopathic function of 9 strains of transmissible gastroenteritis (TGE) virus were examined in primary cells and cell lines such as CPK, IB-RS-2, ESK, and PK-15 originated from porcine kidney and the effects of trypsin on the replication of TGE virus were examined in CPK cells. All strains produced a cytopathic effect and grew well in CPK cells as well as in primary porcine kidney cells. The effect of trypsin on the plaque formation was different from strains. The number of plaques produced by strains TO-163, Ukiha and Niigata increased from 2.6 to 3.52 times when trypsin was present in the medium during incubation at 37 degrees C for 1 hr after adsorption of the virus at 4 degrees C for 2 hr. The plaque sizes of TO-163, h-5, Ukiha and Niigata became larger from 1.4 to 1.7 times, when trypsin was present in the agar MEM overlay.

摘要

在源自猪肾的原代细胞和细胞系（如CPK、IB-RS-2、ESK和PK-15）中检测了9株传染性胃肠炎（TGE）病毒的蚀斑形成、复制及相关细胞病变功能，并在CPK细胞中检测了胰蛋白酶对TGE病毒复制的影响。所有毒株均产生细胞病变效应，在CPK细胞以及原代猪肾细胞中生长良好。胰蛋白酶对蚀斑形成的影响因毒株而异。在4℃吸附病毒2小时后，于37℃孵育1小时期间，当培养基中存在胰蛋白酶时，TO-163、宇治波和新潟毒株产生的蚀斑数量增加了2.6至3.52倍。当琼脂MEM覆盖物中存在胰蛋白酶时，TO-163、h-5、宇治波和新潟毒株的蚀斑大小增大了1.4至1.7倍。