猪传染性胃肠炎（TGE）：体外病毒附着以及聚阴离子和聚阳离子的作用
Transmissible gastroenteritis (TGE) of swine: in vitro virus attachment and effects of polyanions and polycations.
作者信息
Nguyen T D, Bottreau E, Aynaud J M
机构信息
National Institute of Veterinary Research, Bachmai, Hanoi, Vietnam.
出版信息
Vet Microbiol. 1987 Sep;14(4):343-54. doi: 10.1016/0378-1135(87)90026-5.
Four transmissible gastroenteritis virus (TGEV) strains (Purdue-115, D-52, 188-SG and Gep-II) and two cell lines (swine testis-ST and pig kidney-RPD) were used to study virus attachment and cell susceptibility. Virus attachment was partially thermodependent and the rate varied, depending on the strain. Identical TGEV inocula produced a higher plaque number by plaque assay in the swine testis cell line (ST) than in the pig kidney cell line (RPD) but [3H]uridine-labelled virus was found associated equally well with both cell lines. A field TGEV strain (Gep-II), which was unable to multiply in cell cultures, appeared able to inhibit the attachment of radiolabelled cell-passaged virus. Therefore, the susceptibility to TGEV infection was apparently not determined at the virus-to-cell attachment stage. The attachment sites on the cell surface were specific, however, differences in TGEV attachment determinant between strains were not observed. Attachment of all the virus strains tested was enhanced by DEAE-dextran and inhibited by dextran sulfate, poly-L-lysine (PLL), poly-L-alpha-ornithine (PLO) and protamine sulfate.
使用四种传染性胃肠炎病毒（TGEV）毒株（普渡-115、D-52、188-SG和Gep-II）和两种细胞系（猪睾丸-ST和猪肾-RPD）来研究病毒附着和细胞易感性。病毒附着部分依赖于温度，且速率因毒株而异。相同的TGEV接种物通过空斑试验在猪睾丸细胞系（ST）中产生的空斑数高于猪肾细胞系（RPD），但发现[3H]尿苷标记的病毒与两种细胞系的结合效果相同。一种在细胞培养中无法增殖的TGEV野毒株（Gep-II）似乎能够抑制放射性标记的细胞传代病毒的附着。因此，对TGEV感染的易感性显然不是在病毒与细胞附着阶段决定的。然而，细胞表面的附着位点是特异性的，未观察到毒株之间TGEV附着决定因素的差异。所测试的所有病毒毒株的附着均通过DEAE-葡聚糖增强，并被硫酸葡聚糖、聚-L-赖氨酸（PLL）、聚-L-α-鸟氨酸（PLO）和硫酸鱼精蛋白抑制。
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